Acta Scientiarum Naturalium Universitatis Pekinensis

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Localization of Specific Glycoproteins of Sperm Cell Membrane from Zea mays by Electronmicroscopy Immuno-gold Labelling

YANG Zhonghan, CHEN Li, SHAO Linxiang, WANG Jie   

  1. College of Life Science, Peking University, Beijing, 100871, China
  • Received:1999-03-01 Online:2000-03-20 Published:2000-03-20


杨中汉, 陈丽, 邵邻相, 汪洁   

  1. 北京大学生命科学学院,北京,100871

Abstract: Plasma membrane proteins of sperm cells from Zea mays were passed through affinity column, separated by SDS-PAGE and stained using Con A-HRP. The components of 37kD and 39kD glycoproteins from gel were immunized respectively mice and polyclonal antiserums were obtained. Glycoproteins of sperm cell surface can be examined using IgG(rabbit anti mouse)-colloidal gold(15nm) as probe. The results showed that distribution of 37 and 39kD peptides on the plasma membrane surface of sperm cells of maize with visible immunogold particles to be localized. We consider that 37 and 39kD polypeptide were specific plasma membrane glycopeptides of sperm cells. Research of their function is to be continued.

Key words: sperm cell, plasma membrane glycoproteins, affinity chromatography, immunogold labelling, Zea mays

摘要: 用亲和层析,SDS-PAGE及印迹技术,从玉米精细胞中分离纯化质膜蛋白。用ConA-HRP检测获得分子量为37和39kD两种糖蛋白特异成分。用它们分别免疫小鼠得到多克隆抗体,以兔抗鼠IgG胶体金(15nm)为探针,检查此两种蛋白在精细胞质膜上的分布。免疫定位表明,37kD, 39kD多肽是玉米精细胞质膜所特异的。有关此多肽的功能有待进一步研究。

关键词: 精细胞, 质膜糖蛋白, 亲和层析, 胶体金免疫定位, 玉米

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