Cloning and Expression of the Rabbit Growth Hormone cDNA

Acta Scientiarum Naturalium Universitatis Pekinensis

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Cloning and Expression of the Rabbit Growth Hormone cDNA

DONG Yuqing, LI Ronggui, ZHAO Jindong, WU Guangyao

College of Life Sciences, Peking University, Beijing, 100871

Received:1999-01-12 Online:2000-03-20 Published:2000-03-20

兔生长激素cDNA克隆和表达

董宇清, 李荣贵, 赵进东, 吴光耀

北京大学生命科学学院，北京，100871

Abstract

Abstract: The cDNA of preprotein and mature protein of rabbit hypophysiscerebri was obtained. The cDNA of mature growth hormone was cloned in pET-3a, then the recombinant expression vector was transformed into E.coli BL21(DE3 plys). The overexpression was performed and the yield was 40% of the total protein.

Key words: rabbit growth hormone, cDNA clone, expression

摘要： 从兔脑下垂体中的mRNA中，利用合成引物逆转录PCR，获得了前体和成熟生长激素的cDNA，成熟生长激素的cDNA克隆在pET-3a表达载体并转化到大肠杆菌BL21(DE3 plys)中，获得了高效表达，表达量达到大肠杆菌总蛋白的40%。

关键词: 兔生长激素, cDNA克隆, 表达

CLC Number:

Q344⁺.13

DONG Yuqing,LI Ronggui,ZHAO Jindong,WU Guangyao. Cloning and Expression of the Rabbit Growth Hormone cDNA[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

董宇清, 李荣贵, 赵进东, 吴光耀. 兔生长激素cDNA克隆和表达[J]. 北京大学学报（自然科学版）.

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URL: https://xbna.pku.edu.cn/EN/

https://xbna.pku.edu.cn/EN/Y2000/V36/I2/199

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Cloning and Expression of the Rabbit Growth Hormone cDNA

兔生长激素cDNA克隆和表达

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