Acta Scientiarum Naturalium Universitatis Pekinensis

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A Modified Procedure for the Determination of the Gene Copy Number

WANG Ying1, CHEN Laitong2   

  1. 1Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080; 2College of Life Science, Peking University, Beijing, 100871
  • Received:1996-03-20 Online:1996-11-20 Published:1996-11-20



  1. 1中国科学院动物研究所,北京,100080; 2北京大学生命科学学院,北京,100871

Abstract: A modified method for the estimation of gene copy number in genome is proposed. It based upon the size ratio between the cloned gene and its haploid genome. From the size fraction and the amount of genomic DNA used for analysis, the gene copy number could be estimated from the data of dot blot or Southern blot analysis. Using the procedure proposed by this study, the copy numbers of human α1-antitrypsin gene and rat RALRS-1 repetitive sequence were determined.

Key words: gene copy number, haploid genome, human α1-antitrypsin gene, rat RALRS-1 repetitive sequence

关键词: 基因拷贝数, 单倍体基因组, α1-抗胰蛋白酶基因, 大鼠RALRS-1重复序列

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