一株氢氧化细菌的生长条件及其对不同氮源利用的研究

doi:10.13209/j.0479-8023.2021.059

北京大学学报自然科学版 ›› 2021, Vol. 57 ›› Issue (4): 756-764.DOI: 10.13209/j.0479-8023.2021.059

一株氢氧化细菌的生长条件及其对不同氮源利用的研究

陶虎春^†, 谢勇, 张丽娟, 丁凌云, 陈艺贞

深圳市重金属污染控制与资源化重点实验室, 北京大学深圳研究生院环境与能源学院, 深圳 518055

收稿日期:2020-06-01 修回日期:2020-06-13 出版日期:2021-07-20 发布日期:2021-07-20
通讯作者: 陶虎春, E-mail: taohc(at)pkusz.edu.cn
基金资助:
国家自然科学基金(51679002, 51809004)、深圳市基础研究项目(JCYJ20180503182122539)和流域生态工程学学科建设资金(深发改〔2017〕542 号)资助

Growth Conditions of a Hydrogen-Oxidizing Bacterium and Its Utilization of Different Nitrogen Sources

TAO Huchun^†, XIE Yong, ZHANG Lijuan, DING Lingyun, CHEN Yizhen

Shenzhen Key Laboratory for Heavy Metal Remediation and Reclamation, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055

Received:2020-06-01 Revised:2020-06-13 Online:2021-07-20 Published:2021-07-20
Contact: TAO Huchun, E-mail: taohc(at)pkusz.edu.cn

摘要/Abstract

摘要：

从污水处理厂活性污泥中成功地筛选出一株自养型氢氧化细菌(Hydrogen-oxidizing Bacterium, HOB), 命名为Rhodoblastus sp. TH20。以模拟氨氮(NH₄⁺-N)废水作为培养基, 该菌株能够以H₂为能源, CO₂为碳源, 其最适宜的生长条件为25℃、160 rpm和pH=7.0。当初始NH₄⁺-N浓度为100 mg/L时, 菌株在72小时内能有效地去除NH₄⁺-N (>99%)。其中, 77.8%的 NH₄⁺-N被同化为有机氮, 储存在细胞体内, 剩余的转化为气态氮。结果表明: Rhodoblastus sp. TH20具有高效的氨同化能力, 可实现NH₄⁺-N向微生物蛋白的资源转化, 为含NH₄⁺-N污水的资源化处理提供一条新途径。

关键词: 氢氧化细菌, 生长条件, 氮源, 微生物蛋白

Abstract:

An autotrophic hydrogen-oxidizing bacterium, named Rhodoblastus sp. TH20, was successfully enriched and isolated from municipal activated sludge in a domestic wastewater treatment plant. With simulated ammonium-containing wastewater as culture medium, the strain could utilize H₂ and CO₂ as energy and carbon sources respectively and the growth conditions were optimized at 25℃, 160 rpm and pH=7.0. At initial concentration of 100 mg/L, efficient ammonium removal (>99%) was achieved within 72 hours. Up to 77.8% of ammonium was assimilated to organic nitrogen and stored intracellularly, while the rest ammonium was transformed to gaseous N₂. These results indicated that Rhodoblastus sp. TH20 was able to efficiently convert ammonium into microbial protein, thereby providing a sustainable pathway of wastewater treatment.

Key words: hydrogen-oxidizing bacteria, growth condition, nitrogen source, microbial protein

陶虎春, 谢勇, 张丽娟, 丁凌云, 陈艺贞. 一株氢氧化细菌的生长条件及其对不同氮源利用的研究[J]. 北京大学学报自然科学版, 2021, 57(4): 756-764.

TAO Huchun, XIE Yong, ZHANG Lijuan, DING Lingyun, CHEN Yizhen. Growth Conditions of a Hydrogen-Oxidizing Bacterium and Its Utilization of Different Nitrogen Sources[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2021, 57(4): 756-764.

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链接本文: https://xbna.pku.edu.cn/CN/10.13209/j.0479-8023.2021.059

https://xbna.pku.edu.cn/CN/Y2021/V57/I4/756

一株氢氧化细菌的生长条件及其对不同氮源利用的研究

Growth Conditions of a Hydrogen-Oxidizing Bacterium and Its Utilization of Different Nitrogen Sources

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