北京大学学报自然科学版 ›› 2020, Vol. 56 ›› Issue (4): 739-744.DOI: 10.13209/j.0479-8023.2020.036

上一篇    下一篇

不同光质对嗜热蓝细菌Synechococcus sp. PCC6715生长的影响

李凯, 宫一玮, 李兴康, 张艳婷, Maurycy Daroch, 金鹏†   

  1. 北京大学深圳研究生院环境与能源学院, 深圳 518055
  • 收稿日期:2019-04-28 修回日期:2020-04-07 出版日期:2020-07-20 发布日期:2020-07-20
  • 通讯作者: 金鹏, E-mail: jinpeng(at)pkusz.edu.cn
  • 基金资助:
    深圳市协同创新计划国际合作研究项目(GJHZ20180928162210431)资助

Effects of Different Light Qualities on the Growth of Thermophilic Cyanobacteria Synechococcus sp. PCC6715

LI Kai, GONG Yiwei, LI Xingkang, ZHANG Yanting, Maurycy Daroch, JIN Peng†   

  1. School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055
  • Received:2019-04-28 Revised:2020-04-07 Online:2020-07-20 Published:2020-07-20
  • Contact: JIN Peng, E-mail: jinpeng(at)pkusz.edu.cn

PDF

摘要：

使用光合有效光量子数密度(PAR)均为100 μmol/(m2·s)的白光(对照)、红光和蓝光, 对对数生长期的嗜热蓝细菌PCC6715进行培养, 以期探明不同光质对不表达藻红素的蓝细菌光适应生长的影响。结果表明, 在不同光质培养对数生长期PCC6715的过程中, 与白光相比, 干重在蓝光下明显增加, 红光下明显减少; 红、蓝光质对光合色素合成产生影响的开始时间和持续时间有所不同; 最大光能转化效率(Fv/Fm)在蓝光下明显提高, 红光下明显降低, 培养6天后, 红、蓝光条件下的Fv/Fm均趋于稳定。蓝光有利于PCC6715的生长, 红光不利于PCC6715的生长; 同时, PCC6715在红、蓝光质中均产生适应性生长, 藻蓝素的减少使PCC6715在红光中发生适应性生长, 蓝光中PCC6715发生适应性生长是由于藻蓝素的增加。

关键词: 蓝细菌, 嗜热蓝细菌, 光质, 光适应生长, 藻蓝素

Abstract:

 In order to understand the lighting quality effects on the light-adaptive growth of cyanobacteria which does not express phycoerythrin, the thermophilic cyanobacteria PCC6715 in logarithmic growth phase was cultured by using white light (control), red light, and blue light with photosynthetically effective photon quantum density (PAR) of 100 μmol/(m2·s). Compared with the growth characteristics of PCC6715 under white light conditions, the changes in the measured and analyzed values of PCC6715 under other light qualities were as follows: the dry weight of PCC6715 significantly increased under blue light and significantly reduced under red light; the red and blue light have different effects on the start time and duration of the photosynthetic pigment synthesis of PCC6715; the maximum light energy conversion efficiency (Fv/Fm) of PCC6715 significantly increased under blue light and decreased significantly under red light; after 6 days of culture, the Fv/Fm of PCC6715 under red and blue light conditions tends to stable. In conclusion, the blue spectrum lighting promotes the growth of PCC6715, while red light inhibits the growth of PCC6715. At the same time, PCC6715 produces light-adaptive growth under both red and blue light, the reduction of phycocyanin content caused light-adaptive growth of PCC6715 in red light, and the light-adaptive growth of PCC6715 in blue light was due to the increase of phycocyanin content.

Key words: cyanobacteria, thermophilic cyanobacteria, light quality, light-adaptive growth, phycocyanin

版权所有 © 《北京大学学报（自然科学版）》编辑部
技术支持：北京玛格泰克科技发展有限公司