关键词: 大气压低温等离子体, 活性氧, 金黄色葡萄球菌

Abstract: The authors carried out the reactive species and sterilization efficacy of a direct current atmospheric pressure Ar+O₂ (2%) cold plasma micro jet. Electron spin resonance (ESR) spectroscopy and other methods were employed to investigate the reactive species of the plasma. The plasma micro jet was submerged in Staphyloccocus aureus suspensions for the study of its sterilization effects. Two types of reactive species produced in the solution were monitored by ESR spectroscopy directivity, namely hydroxyl radical (OH?) and singlet oxygen (¹O₂). Superoxide anion radical (O₂^-?) was also proved in the system directly. O₃ and H₂O₂ in the system were also analysed quantitatively. With 10 min Ar+O₂(2%) cold plasma micro jet treatment, a quick increase of the percent inactivation from 0 to 99.9% was achieved. Non-thermal atmospheric pressure microplasma micro jet can generate enormous reactive species and can efficiently inactivate S. aureus in water. The possible mechanism is that the reactive species induced the cell generate superoxide membrane lipid, protein and DNA through various oxidation progress. Non-thermal atmospheric pressure plasma micro jet has enormous potential clinical application.

Key words: atmospheric pressure cold plasma, reactive oxygen species, Staphyloccocus aureus