Acta Scientiarum Naturalium Universitatis Pekinensis
WANG Shanlin1，ZHANG Jianbo1，4，WANG Weijing1，YANG Yuxiang2，3
Fe3O4-asorption-gelatin-entrapping method for immobilization of horseradish peroxidase was established. Immobilization efficiency of this carrier was compared with that of sodium alginate, gelatin and open pore gelatin cube. It was found that higher efficiency could be achieved by Fe3O4-asorption-gelatin-entrapping method. The stabilities of different immobilized enzymes and free HRP were also compared, and the stability of immobilized HRP with Fe3O4-gelatin as carrier was considerably higher than the stabilities of other enzymes. Effects of concentration of glutaraldehyde and gelatin, crosslinking time, dosage of HRP and Fe3O4 on the activity of immobilized HRP were studied. The results of optimal conditions of immobilization were as follows: the optimal ratio of dosage of HRP and Fe3O4 was about 95 u HRP/g Fe3O4, the optimal ratio of Fe3O4 and gelatin was 1.0g Fe3O4/10mL 10％～20％ gelatin, the optimal glutaraldehyde concentration was 0.5% and the optimal crosslinking time was 30min. On this condition, the activity of immobilized HRP was about 1.1u/g and the recovery of enzyme activity was around 15%. Repetitive use of immobilized HRP for the elimination of PCP was examined and stable efficiency of PCP degradation was achieved.
辣根过氧化物酶固定效果的影响。结果表明，固定化反应的最佳条件如下：最佳给酶量与Fe3O4用量比例约为95u HRP/g Fe3O4，最佳Fe3O4用量与凝胶比例为1.0g
WANG Shanlin,ZHANG Jianbo,WANG Weijing,YANG Yuxiang. Preparation and Application of Horseradish Peroxidase Immobilized in Fe3O4-Asorption-Gelatin-Entrapping Method[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.
王杉霖,张剑波,王维敬,杨宇翔. 四氧化三铁吸附包埋固定辣根过氧化物酶及其应用[J]. 北京大学学报（自然科学版）.
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