Upland Rice Transformation

Abstract

Abstract: Herbicide-resistant transgenic upland rice plants were recovered using biolistic method. Upland rice suspension cells and immature embryos were transformed by particle bombardment with a plasmid containing herbicide resistant bar gene. One week after bombardment, the bombarded cells were transferred to N6 medium with phosphinothricin (PPT) for selection. Fertile transgenic plants were regenerated from PPT-resistant calli. Integration of bar gene was confirmed by Southern blot hybridization. Resistance of R0 transgenic plants to herbicide PPT was shown by Basta (commercial form of PPT) applications in the greenhouse. Inheritance of bar gene was demonstrated by PCR analysis of R1 transgenic plants.

Key words: Upland rice, BAR, transformation, phosphinothricin

摘要： 以旱稻丹粳旱5-55、6-24、6-37(Oryza sativa L. var japonica)的悬浮细胞及未成熟胚为受体材料，采用基因枪法将含BAR基因的pDM302质粒DNA导入旱稻细胞中，经PPT筛选获得抗性愈伤组织，筛选出的愈伤组织在分化培养基上再生出完整的旱稻转基因植株。Southern分子杂交分析表明，外源BAR基因已整合到水稻基因组内，抗除草剂试验结果表明，转化植株对0.01%Basta(有效成分为PPT)有一定程度的抗性，说明外源BAR基因已在旱稻细胞内正确表达。90%的转基因植株可育并已结实，部分R1代植株 PCR扩增结果证明外源BAR基因已遗传给后代。

关键词: 旱稻, 基因枪转化, BAR, PPT

CLC Number:

YU Zhihua,CHEN Minyong,GU Hongya,PAN Naisui,CHEN Zhangliang,LI Yan. Upland Rice Transformation[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

于志华,陈敏勇,顾红雅,潘乃禾遂,陈章良,李岩 . 抗除草剂旱稻转基因植株的获得[J]. 北京大学学报（自然科学版）.

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Upland Rice Transformation

抗除草剂旱稻转基因植株的获得

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