北京大学学报(自然科学版)

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利用里氏木霉生物转化制备黄姜薯蓣皂甙元的清洁新工艺

朱余玲1,黄文2,刘葳1,倪晋仁1   

  1. 1. 北京大学环境工程系, 教育部水沙科学重点实验室,北京 100871; 2.四川大学华西医院肿瘤生物治疗国家重点实验室,成都 610041;

  • 收稿日期:2009-06-01 出版日期:2010-07-20

A Green Extraction Process of Diosgenin from Dioscorea zingiberensis Tubers with Trichoderma reesei

ZHU Yuling1, HUANG Wen2, LIU Wei1, NI Jinren1   

  1. 1. Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University, Beijing 100871; 2. State Key Laboratory of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041;

  • Received:2009-06-01 Published:2010-07-20

摘要: 基于清洁生产和资源综合利用的理念, 提出一种从盾叶薯蓣根茎中提取薯蓣皂甙元的新工艺。该工艺由两部分组成, 第一部分利用淀粉酶和糖化酶分离黄姜根茎中的淀粉, 第二部分利用里氏木霉转化糖渣 中的皂苷制备薯蓣皂甙元。研究表明, 与直接酸水解相比, 新工艺可以提取约 98.7%薯蓣皂甙元, 并以还原糖 的形式回收98. 0% 淀粉, 降低生产废水中 99. 4%COD, 100% SO2-4 和100% 酸。在酸水解废水中检测到24 种有机物, 其中邻苯二甲酸二丁酯和邻苯二甲酸-2-乙基己酯属于美国环保局优先控制污染物。而新工艺废水中仅检测到两种有机物(17β-羟基雄甾-5-烯-3-酮和邻苯二甲酸二异丁酯) 。本研究为皂素清洁生产提供了一种新方法。

关键词: 薯蓣皂甙元, 皂苷, 里氏木霉, 黄姜

Abstract: With the concept of resource efficiency and cleaner production, a green extraction process of diosgenin from Dioscorea zingiberensis tubers was proposed. This method contained of excluding starch from raw material and bioconverting the saponins in the residue by Trichoderma reesei. Compared to acid hydrolysis, 98.7% of diosgenin could be extracted from plant tubers through the new process, with 98.0% of starch recovered in the form of sugar. Consequently, 99.4% ofCOD, 100% of SO2-4 and 100% of acid were reduced in processing wastewater. In addition, acid hydrolysis by-products were greatly limited, e.g. two priority pollutants [Bis( 2-ethylhexyl) phthalate and Di-n-butyl phthalate], which are specified by United States Environmental Protection Agency, typically detected in Dioscorea zingiberensis wastewater from conventional acid hydrolysis process, disappeared automatically in the proposed cleaner process.

Key words: diosgenin, saponins, Trichoderma reesei, Dioscorea zingiberensis

中图分类号: 

  • X506