白皮松形成层活动周期中过氧化物酶和酯酶同工酶的变化

北京大学学报（自然科学版）

白皮松形成层活动周期中过氧化物酶和酯酶同工酶的变化

崔克明¹, 张仲鸣¹, 李举怀²

¹北京大学生命科学学院，北京，100871; ²北京市农林科学院作物研究所，北京，100081

收稿日期:1996-10-03 出版日期:1997-03-20 发布日期:1997-03-20

Changes of Peroxidase and Esterase Isozyme during Periodicity of Cambial Activity in Pinus bungeana

CUI Keming¹, ZHANG Zhongming¹, LI Juhuai²

¹College of Life Sciences, Peking University, Beijing, 100871; ²Crops Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100081

Received:1996-10-03 Online:1997-03-20 Published:1997-03-20

摘要/Abstract

摘要： 白皮松(Pinus bungeana Zucc.)枝条不同组织中过氧化物酶(POD)和酯酶(EST)同工酶的酶谱也不同，而且每种组织中这两种酶的同工酶酶谱随形成层活动周期的变化而变化。在休眠期，形成层区域过氧化物酶的同工酶酶带最多，这时周皮和韧皮部中酯酶同工酶酶谱在正极端出现一些酶带，这可能与抗寒有关。活动开始时形成层区域负极端出现一特异酯酶酶带，可能与形成层开始分化有关。活动期中，周皮、韧皮部和木质部中酯酶的正极端也有特异性表达的酶带，这些酶带与韧皮部和木质部的分化密切相关。一些过氧化物酶同工酶酶带在形成层恢复活动时消失，到休眠期又出现，这可能与IAA(3-吲哚乙酸)水平的变化有关。

关键词: 白皮松, 形成层活动周期, 酯酶同工酶, 过氧化物酶同工酶

Abstract: The seasonal changes of peroxidase and estarase isozyme in various tissues of Pinus bungeana were mere related to the annual cycle of cambial activity, and to types of the tissues. During cambial dormancy period, number of peroxidase isozyme bands in cambiumregion reached the maximum. In the time, some cationic estarase isozyme bands occured in the periderm and phloem, these could be related to a protective against frost. When reactitivity of cambium, two cathodic estarase isozyme bands occured in the cambial region, these could be related to enlarging of the cambial cells and formation of new xylem and phloem. During the cambial activity period, othercationic estarase isozyme bands occured in the periderm, xylem andphloem, these could be closely related to the differentiation of phloemand xylem. Some perioxidase isozyme bands disappeared during cambial reactivity, and occured during cambial dormancy period, these could be related to the changes of the level of IAA.

Key words: Pinus bungeana Zucc., periodicity of cambial activity, peroxidase isozyme, estarase isozyme

中图分类号:

Q944.33

崔克明, 张仲鸣, 李举怀. 白皮松形成层活动周期中过氧化物酶和酯酶同工酶的变化[J]. 北京大学学报（自然科学版）.

CUI Keming,ZHANG Zhongming,LI Juhuai. Changes of Peroxidase and Esterase Isozyme during Periodicity of Cambial Activity in Pinus bungeana[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

[1]	王震,崔克明, 李举怀. 构树去木质部后TIBA对组织再生的影响及其间过氧化物酶和酯酶同工酶的变化[J]. 北京大学学报（自然科学版）, 1999, 35(6): 750-759.
[2]	罗立新,崔克明,李正理, 李举怀. 杜仲形成层活动周期及过氧化物酶和酯酶同工酶的变化[J]. 北京大学学报（自然科学版）, 1999, 35(2): 209-216.