北京大学学报(自然科学版)

共培养法获得MC15昆明嵌合鼠

何维, 吴鹤龄   

  1. 北京大学生命科学学院,北京,100871
  • 收稿日期:1996-09-23 出版日期:1997-03-20 发布日期:1997-03-20

Production of MC15-Kunming Chimeras by Culture

HE Wei, WU Heling   

  1. College of Life Sciences, Peking University, Beijing, 100871
  • Received:1996-09-23 Online:1997-03-20 Published:1997-03-20

摘要: 嵌合鼠的获得是通过ES细胞途径得到转基因小鼠的关键环节。以MC15为供ES细胞,用昆明白品系小鼠8细胞期的胚胎为受体胚胎,采用共培养方法我们获得了2只毛色嵌合鼠。通过与囊胚显微注射法比较,表明这种方法是方便可行的。

关键词: ES细胞, 8细胞胚, 共培养, 嵌合鼠

Abstract: Two MC15-KunMing chimeras were produced by a new method that involves the simple coculture of eight-cell embryos on a lawn of ES cells. Compared with microinjection blastocyst, this methodis feasible.

Key words: ES cell, eight-cell embryo, coculture, chimeras

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