This study successfully isolated and screened a microbial strain with efficient alginate-degrading capability from seaweed, utilizing alginate as the sole carbon source in a selective culture medium. The strain was systematically identified and subjected to bioinformatics analysis through physiological and biochemical characterization, 16S rDNA sequence analysis, and whole-genome sequencing to uncover potential alginate lyase-related genes. Additionally, the enzyme production conditions for the strain were optimized using single-factor experiments, Plackett-Burman design, and response surface methodology. Morphological observations, physiological and biochemical trait analysis, and 16S rDNA gene sequence identification confirmed the strain to be of the genus Cobetia. The full length of the strain’s genome is 4136490 base pairs, with a GC content of 62.48%. Genome prediction encoded 3454 protein-coding genes, 70 tRNA genes, and 12 rRNA genes. Nucleotide consistency analysis was subsequently performed and it was named Cobetia marina 29. Nucleotide consistency analysis was subsequently performed and it was named Cobetia marina 29. Functional annotation using the GO, KEGG, COG, and CAZy databases yielded 2487, 2271, 2910, and 145 annotated functional genes, respectively. Among these, three genes encoded alginate lyases and were classified into the PL17 and PL7 families. Finally, the cultivation parameters were refined, and under the optimal conditions for enzyme production, the enzyme activity was enhanced to 179.29 U/mL, which represented a 53.6% increase compared with the levels prior to optimization. The findings of this study provided a new microbial resource for the biodegradation of alginate and laid the foundation for subsequent industrial applications.

以褐藻胶作为选择性培养基的唯一碳源, 从海藻中分离筛选出一株具有良好褐藻胶降解能力的微生物菌株。通过生理生化鉴定、16S rDNA序列分析以及全基因组测序, 对该菌株进行系统的鉴定和生物信息学分析, 揭示可能的褐藻胶裂解酶相关基因。此外, 通过单因素试验、Plackett-Burman设计和响应面法对该菌株的产酶条件进行优化。形态学观察、生理生化特征分析及16S rDNA基因序列鉴定结果确认该菌株为科贝特氏菌属。该菌株基因组全长为4136490 bp, GC含量为62.48%。基因组预测编码3454个蛋白编码基因, 70个tRNA基因和12个rRNA基因。随后进行核苷酸一致性分析, 将其命名为Cobetia marina 29。在基因功能注释方面, 通过GO, KEGG, COG和CAZy数据库, 分别识别出2487, 2271, 2910和145个具有特定功能的基因, 其中3个基因编码褐藻胶裂解酶, 分别属于PL17和PL7家族。最后, 对培养条件进行优化, 在最佳产酶条件下, 酶活性提高至179.29 U/mL, 比优化前提高53.6%。研究结果为褐藻胶的生物降解提供了新的菌种资源, 并为后续工业化应用奠定基础。