Abstract:

The fungal strains were obtained by single colony isolation technique from the soil of the world’s unique proved independent selenium deposit. The anti-tumor activity was assayed by MTT method using MDA-MB-231cells, Hela cells and SMMC-7721 cells, respectively, together with morphological observation of these cells under inversed light microscope. The 73 terrestrial fungal strains were isolated from the soil samples collected from 6 different areas in Enshi area of HuBei Province, China. Among them, 32 strains produced fermentation products obviously inhibiting the Hela cells, 13 strains inhibiting the MDA-MB-231 cells and 9 strains inhibiting the SMMC-7721 cells with IC₅₀ value less than 90 µg/ml. These antitumor-metabolite-producing strains have provided fungal strains for further studies on the bioactive metabolites.

Key words: fungi, selenium mining area, terrestrial fungus, isolation and cultivation, anti-tumor activity

摘要：

利用单菌落挑选与划线培养技术, 从硒矿区土壤样品中分离纯化真菌。通过摇床发酵培养, 提取制备发酵样品。采用 MTT 法, 用 MDA-MB-231 细胞、Hela 细胞、SMMC-7721 细胞测试发酵样品的抗肿瘤活性。从采自湖北恩施全球唯一独立硒矿床区的 6 个不同土样中分离真菌73株。其中, 对人宫颈癌Hela细胞的IC₅₀小于90 µg/mL的菌株32株, 对人乳腺癌MDA-MB-231细胞的IC₅₀小于90 µg/mL的菌株13株, 对人肝癌SMMC-7721细胞的IC₅₀小于90 µg/mL的菌株9株。这些活性菌株为筛选抗肿瘤天然产物提供了良好的药源菌株。

关键词: 真菌, 硒矿床区, 陆生真菌, 分离培养, 抗肿瘤活性