Abstract:

To study the effect of long-term mining on the microbes in the environment, the soil sampled from Dexing copper ore deposit and its surrounding environment was chosen to be the object of this study. The distribution characteristics of microorganisms in soil were analyzed, and the spatial dependence of between microorganisms and heavy metal elements in surface soil was discussed by using microbial culture method, nucleic acid sequence analysis method and transmission electron microscopy. The experimental results showed that 13 kinds of microbes were isolated from the soil samples in Dawuhe-Gukou riverbank and Zhujiacun. The bacteria isolated from the soil samples in Dawuhe-Gukou riverbank and Zhujiacun had a certain similarity in type and distribution layer; however, to fungi, the similarity between Dawuhe-Gukou riverbank and Zhujiacun was not obvious. The microbial distribution and heavy metal element content in the soil profile had a certain relationship, but not to a simple linear relation, also influenced by various environmental factors.

Key words: soil, microorganism, heavy metal element, distribution

摘要：
摘要 为了研究长期采矿对矿区环境微生物的影响, 以江西德兴铜矿周围环境土壤为研究对象, 利用微生物
培养法、核酸序列分析法、透射电镜等手段, 分析和研究德兴铜矿地区土壤微生物的分布特征, 并探讨表层
土壤微生物与重金属的空间依存关系。研究结果表明, 在实验室条件下, 大坞河?沽口河岸和祝家村土壤中
可培养微生物共有13 种, 大坞河–沽口河岸和祝家村土壤中的细菌在种类和分布层位上具有一定的相似性;
但在真菌方面, 大坞河–沽口河岸和祝家村土壤之间相似性不明显。大坞河–沽口河岸与祝家村土壤剖面中微
生物的分布与重金属元素含量具有一定的相关性, 但并非为简单的线性关系, 还受到多种环境因素的影响。

关键词: 土壤, 微生物, 重金属元素, 分布