Acta Scientiarum Naturalium Universitatis Pekinensis

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Screening of Lead-Tolerant Microbes and Preliminary Study on Their Removal Capability to Lead

YANG Liang, HAO Ruixia, WU Feng, XIAO Yuxiong   

  1. The Laboratory of Orogenic Belts and Crustal Evolution, School of Earth and Space Sciences, Peking University, Beijing 100871;
  • Received:2011-12-09 Online:2012-11-20 Published:2012-11-20



  1. 北京大学地球与空间科学学院造山带与地壳演化重点实验室, 北京100871;

Abstract: Four lead-tolerant strains were isolated from lead-contaminated soil around the lead-zinc ore deposit. All strains could grow in nutrient solution containing 100?1000 mg/L Pb(II). The removal rates of Pb(II) by Pb-X-1, Pb-Z-3 and Pb-Z-4 were 78.31%, 77.8% and 87% respectively in the following conditions: pH was 6, 25℃, 160 r/min, initial Pb2+ concentration was 600 mg/L. The Pb(II) removal rate by Pb-X-2 was higher than 95%, when initial Pb(II)concentration was 100?400 mg/L. The result of 16S rRNA sequencing indicates that Pb-X-2 belonged to Stenotrophomonas maltophilia. The removal mechanism of Pb(II) removal by Pb-X-2 was mainly cellular surface adsorption and intracellular enrichment.

Key words: lead, remove, Stenotrophomonas maltophilia

摘要: 从铅锌尾矿厂周围土壤样品中分离出4株耐铅菌。4株耐铅菌均能在Pb2+浓度范围为100~1000 mg/L的培养液中生长。在pH 6, 25℃, 160 r/min及初始Pb2+浓度为600 mg/L时, 菌株Pb-X-1, Pb-Z-3和Pb-Z-4的Pb2+去除率分别为78.31%, 77.8%和87%。菌株Pb-X-2在初始Pb2+浓度为100~400 mg/L时, 去除率均高于95%。16S rRDA基因序列分析表明, Pb-X-2属于嗜麦芽寡养单胞菌。菌株Pb-X-2对溶液中铅离子的去除机理为细胞表面吸附与胞内富集。

关键词: 铅, 去除, 嗜麦芽寡养单胞菌

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