Abstract: Four lead-tolerant strains were isolated from lead-contaminated soil around the lead-zinc ore deposit. All strains could grow in nutrient solution containing 100?1000 mg/L Pb(II). The removal rates of Pb(II) by Pb-X-1, Pb-Z-3 and Pb-Z-4 were 78.31%, 77.8% and 87% respectively in the following conditions: pH was 6, 25℃, 160 r/min, initial Pb²⁺ concentration was 600 mg/L. The Pb(II) removal rate by Pb-X-2 was higher than 95%, when initial Pb(II)concentration was 100?400 mg/L. The result of 16S rRNA sequencing indicates that Pb-X-2 belonged to Stenotrophomonas maltophilia. The removal mechanism of Pb(II) removal by Pb-X-2 was mainly cellular surface adsorption and intracellular enrichment.

Key words: lead, remove, Stenotrophomonas maltophilia

摘要： 从铅锌尾矿厂周围土壤样品中分离出4株耐铅菌。4株耐铅菌均能在Pb²⁺浓度范围为100~1000 mg/L的培养液中生长。在pH 6, 25℃, 160 r/min及初始Pb²⁺浓度为600 mg/L时, 菌株Pb-X-1, Pb-Z-3和Pb-Z-4的Pb²⁺去除率分别为78.31%, 77.8%和87%。菌株Pb-X-2在初始Pb²⁺浓度为100~400 mg/L时, 去除率均高于95%。16S rRDA基因序列分析表明, Pb-X-2属于嗜麦芽寡养单胞菌。菌株Pb-X-2对溶液中铅离子的去除机理为细胞表面吸附与胞内富集。

关键词: 铅, 去除, 嗜麦芽寡养单胞菌