Observation of Chromosome Structure in Allium CepaCells by Means of Electron Microscopy

Abstract

Abstract: NAMA-Ur is a specific DNA stain method that has been developed in recent years. In the Allium cepa cells only the chromatin or chromosomes are heavily stained, the other components are bleached. After the chromosomes are stained by NAMA-Ur, we have observed that the highest order structure of chromosomes is helical；while the secondary structure is radically folding. The DNA fibres with a diameter of 25～30nm have radically folded into chromatid fibres in a diameter of approximately 250nm. The above results present new evidence to support the radial loop and coils coexist mode of chromosome. This mode may more possibly accord with the real chromosome structure.

Key words: DNA specific staining, NAMAUr method, chromosome structure, ALLium cepa

摘要： 染色体经DNA特异染色后，观察到其最高层次结构是螺旋的，而次级结构存在着放射环结构，即由直径为25～30nm的DNA纤丝放射状折叠排列成约250nm左右的染色单体丝；染色单体丝进一步螺旋形成中期染色体结构。这些观察结果和染色体的螺旋与放射环共存模型非常相似。这个模型应更接近于洋葱染色体真实结构。

关键词: DNA特异染色, NAMA-Ur方法, 放射环结构, 洋葱染色体

CLC Number:

TAO Wei,LIU Xiaoling,ZHAO Yun,LU Zhigang,ZHAI Zhonghe. Observation of Chromosome Structure in Allium CepaCells by Means of Electron Microscopy[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

陶伟,刘晓玲,赵允,卢智刚,翟中和. 洋葱染色体螺旋与放射环结构的电镜观察[J]. 北京大学学报（自然科学版）.

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Observation of Chromosome Structure in Allium CepaCells by Means of Electron Microscopy

洋葱染色体螺旋与放射环结构的电镜观察

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