Observation of Chromosome Structure in Allium CepaCells by Means of Electron Microscopy

Abstract

Abstract: NAMA-Ur is a specific DNA stain method that has been developed in recent years. In the Allium cepa cells only the chromatin or chromosomes are heavily stained, the other components are bleached. After the chromosomes are stained by NAMA-Ur, we have observed that the highest order structure of chromosomes is helical；while the secondary structure is radically folding. The DNA fibres with a diameter of 25～30nm have radically folded into chromatid fibres in a diameter of approximately 250nm. The above results present new evidence to support the radial loop and coils coexist mode of chromosome. This mode may more possibly accord with the real chromosome structure.

Key words: DNA specific staining, NAMAUr method, chromosome structure, ALLium cepa

摘要： 染色体经DNA特异染色后，观察到其最高层次结构是螺旋的，而次级结构存在着放射环结构，即由直径为25～30nm的DNA纤丝放射状折叠排列成约250nm左右的染色单体丝；染色单体丝进一步螺旋形成中期染色体结构。这些观察结果和染色体的螺旋与放射环共存模型非常相似。这个模型应更接近于洋葱染色体真实结构。

关键词: DNA特异染色, NAMA-Ur方法, 放射环结构, 洋葱染色体

CLC Number:

TAO Wei,LIU Xiaoling,ZHAO Yun,LU Zhigang,ZHAI Zhonghe. Observation of Chromosome Structure in Allium CepaCells by Means of Electron Microscopy[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

陶伟,刘晓玲,赵允,卢智刚,翟中和. 洋葱染色体螺旋与放射环结构的电镜观察[J]. 北京大学学报（自然科学版）.

Add to citation manager EndNote|Ris|BibTeX

URL: https://xbna.pku.edu.cn/EN/

https://xbna.pku.edu.cn/EN/Y2000/V36/I6/795

[1]	CHEN Li,TENG Junlin. Transportation of mRNAin Dendrites [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2009, 45(6): 1075-1080.
[2]	CHEN Danying,SHU Hongbing,ZHAI Zhonghe. Signal Transduction of Apoptosis and NF-κB Activation [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2006, 42(2): 141-145.
[3]	YANG Baicheng,GUO Zhenquan,HUANG Yixiu,ZHANG Fengying,ZHU Shenggeng. The Anti-tumor Role of MTS1 Mediated by Adenovirus [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2003, 39(6): 804-808.
[4]	HU Guoqun,GAO Chongming. The Evidence for the Existence of Intermediate-like Filaments in Carchesium polypinum [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2003, 39(2): 178-181.
[5]	GUO Yan,SUN Yuhui,WEI Chaoliang,DING Mingxiao,CHEN Jianguo. Dynamic Association of Histone H2A and Chromatin [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2003, 39(2): 182-186.
[6]	GAO Yunfei,ZHOU Jianjun,CHEN Xi,ZHAO Yun,SU Xiaoqin,LIU Cheng,ZHAI Zhonghe. Super-High-Concentration of Glucose Can Accelerate the Senescent Process of Human Diploid Fibroblast Cells [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2002, 38(4): 572-575.
[7]	CHEN Qiushi,GAO Chongming. A Preliminary Investigation on the Lamin-like Proteins in Paramecium [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2002, 38(2): 276-282.
[8]	FENG Lei,GAO Chongming,LI Yinzhen. The Microtubule Organization Center (MTOC) and the Construction of Microtubule System in Euglena gracilis [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2001, 37(6): 760-769.
[9]	GAO Chongming,CHEN Qiushi,FENG Lei,LI Yingzhen. Intermediate-like Filaments in Paramecium A Preliminary Investigation with Immunological Methods [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2001, 37(6): 791-796.
[10]	ZHU Aizhi,WANG Xiangyun,JIN Shan,GUO Zhenquan. Study of Tea Polyphenol on the Reverse of Carcinoma Cell Lines' Multidrug Resistance [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2001, 37(4): 496-501.
[11]	FENG Lei,GAO Chongming,LI Yinzhen. Like-Intermediate Filament System in Euglena gracilis [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1999, 35(4): 503-508.
[12]	ZHU Long,LI Yuanzong,CI Yun xiang. The Studies on the Respiratory Burst Changes with Age in Rabbit Peritoneal Macrophage with Chemiluminescence Method [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1999, 35(2): 172-177.
[13]	GAO Chongming,LI Yixiu,CHENG Qiansheng,ZHOU Xiaobo. Genetic Analysis of Tetrahymena by Diversity Sequence Clustering Algorithm [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1998, 34(6): 765-769.
[14]	ZHAO Dazhong,ZHAO Yun,CHEN Danying,SUN Yingli,ZHAI Zhonghe,ZHAI Junhui,WANG Guoli. A Study on Homological Analysis of Intermediate Filament Proteins [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1998, 34(6): 770-774.
[15]	JIANG Zhengfan,ZHU Shan,ZHAI Zhonghe. In Vitro Induction of Apoptosis in Purified Nuclei and A 27 kD Nuclease Activated in Xenopus Egg Extracts [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1998, 34(2): 324-331.

Observation of Chromosome Structure in Allium CepaCells by Means of Electron Microscopy

洋葱染色体螺旋与放射环结构的电镜观察

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics