Abstract: Light microtechnique and SA-β-galactosidase method was used to study the effects of super-high-concentration of glucose on the senescence of human diploid fibroblast 2BS cells, ROS and the membrane potential of mitochondria during this process were measured. Our results showed that 200 mmol/L of glucose inhibited the growth of 2BS cells, led to the changes of reactive oxygen species (ROS) and decrease of mitochondrial membrane potential,and caused senescence of 2BS cells rapidly. It supports the hypothesis of oxidative damage of senescence. Moreover it is a better system for the study of the effects of ROS during the process of replicative senescence.

Key words: glucose, cellular senescence, HDF, ROS, flow cytometry

摘要： 利用光学显微镜观察和酸性β-半乳糖苷酶染色技术研究了高浓度葡萄糖对人二倍体成纤维细胞2BS细胞衰老进程的影响，并用流式细胞仪检测了此过程中活性氧和线粒体膜电位差的变化。结果表明：200 mmol/L的葡萄糖对2BS细胞有生长抑制作用，能引起活性氧含量的变化，导致线粒体膜电位差显著下降，并诱导了细胞的衰老。这为氧化损伤假说提供了新的证据，并为研究活性氧和复制衰老之间的关系提供了较好的体系。

关键词: 葡萄糖, 细胞衰老, 人二倍体成纤维细胞, 活性氧(ROS), 流式细胞术