Cloning, Sequencing of Pea cop1 cDNA and Its Expression in E.coli

Abstract

Abstract: Existing evidences show that cop1 (Constitutive Photomorphogenic 1) is a key photomorphogenic repressor in Arabidopsis thaliana. The cDNA of cop1 gene in pea (Pisum sativum) was cloned from a pea cDNA library with Arabidopsis thaliana cop1 cDNA as a probe. Sequencing analysis revealed that its full length was 2863bp, including 604bp of 5' untranslated region, 243 bp of 3' untranslated region and 2016bp coding region for 672 amino acids. Pea cop1 gene was highly expressed in E.coli. Homology analysis of cop1 from Arbidopsis thaliana, pea and tomato revealed that cop1 might be an evolutionarily very conserved protein.

Key words: pea, cop1, cDNA, cloning, expression

摘要： 以拟南芥cop1 cDNA为探针，从豌豆(Pisum sativum) cDNA文库中克隆到了豌豆cop1 cDNA。序列分析表明，它全长为2863bp，其中包括604bp 5′非编码区、243bp 3′非编码区和2016bp编码区，编码672个氨基酸。在大肠杆菌中实现了豌豆cop1基因的高效表达。对拟南芥、豌豆和番茄3种植物cop1的序列同源性比较表明，cop1可能是一种进化上很保守的蛋白质。

关键词: 豌豆, cop1, cDNA, 克隆, 表达

CLC Number:

ZHAO Li,DUAN Xueyan,WANG Chunxia,WU Xiangyu. Cloning, Sequencing of Pea cop1 cDNA and Its Expression in E.coli[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

赵丽, 段学彦, 王春霞, 吴相钰. 豌豆cop1 cDNA的克隆、序列分析及其在大肠杆菌中的表达[J]. 北京大学学报（自然科学版）.

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URL: https://xbna.pku.edu.cn/EN/

https://xbna.pku.edu.cn/EN/Y1999/V35/I4/453

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Cloning, Sequencing of Pea cop1 cDNA and Its Expression in E.coli

豌豆cop1 cDNA的克隆、序列分析及其在大肠杆菌中的表达

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