In 2020, Environmental DNA Metabarcoding was used to study the fish diversity in the Huangjinxia section of the upper Hanjiang River, and the historical survey data were compared to construct a fish list in the Huangjinxia section of the upper Hanjiang River. A total of 20 fish species were detected from 9 sampling sites, accounting for 45.45% of the list; Cyprinus_carpio, Carassius_auratus, Distoechodon_tumirostris and Squalidus_argentatus are the dominant species. The Shannon index of fish in the upper and lower reaches of the Huangjinxia was significantly different (P < 0.01, n=9), and the Shannon index of fish in the upper reaches was significantly larger than that in the lower reaches. The main reason for the spatial differences in fish diversity is the Huangjinxia hydraulic hub. The fish composition detected by environmental DNA was similar to that obtained by traditional methods. As an emerging biomonitoring tool, environmental DNA macrobarcoding technology can complement traditional fish monitoring methods to rapidly detect fish diversity and its spatial distribution in the upper reaches of the Hanjiang River. 

2020年, 采用环境DNA宏条形码对汉江上游黄金峡鱼类多样性进行研究, 并对比历史调查数据, 构建汉江上游黄金峡段鱼类名录。从9 个采样点中共检测出20种鱼类, 占名录的45.45%; 鲤鱼(Cyprinus_carpio)、鲫鱼(Carassius_auratus)、圆吻鲴(Distoechodon_tumirostris)和银鮈(Squalidus_argentatus)为优势种; 黄金峡上、下游Shannon指数存在显著性差异(P<0.01, n=9), 上游鱼类Shannon指数明显大于下游, 黄金峡水利枢纽是造成鱼类多样性空间差异的主要原因。环境DNA检测出的鱼类组成与过去传统方法监测的结果相近。环境DNA宏条形码技术是一种新兴的生物监测手段, 可以辅助传统鱼类监测方法, 快速检测汉江上游鱼类多样性及其空间分布。