The 16S rRNA Illumina Miseq high-throughput sequencing technology was used to analyze the species composition of archaea in the Danjiangkou reservoir area and the downstream Hanjiang River. The structure of ammonia-oxidizing archaea (AOA) and methanogenic archaea that dominated the water and sediments in the upstream and downstream dam was analyzed. The results showed that the structure of the dominant species was affected by the samples difference, which could be explained by the aerobic preference of the ammonia-oxidizing archaea and the anaerobic properties of the methanogenic archaea. According to the network analysis, there was a significant correlation between the ammonia-oxidizing archaea and the methanogenic archaea in the upstream of the Danjiangkou reservoir. Because of the operation of the Danjiangkou reservoir, the abundance of ammoniaoxidizing archaea in the water and sediments of downstream of the dam was reduced, and the abundance of methanogenic archaea in the sediments was relatively high. The relation between them was not obvious.

采用16S rRNA Illumina Miseq高通量测序技术, 分析丹江口水库库区及汉江下游古菌物种组成, 并对大坝上、下游水体与沉积物中占优势的氨氧化古菌(Ammonia-oxidizing archaea, AOA)和产甲烷古菌(Methanogenic archaea)群落结构进行分析。结果表明, 优势种群落结构组成受到水体与沉积物样本差异的影响, 可由氨氧化古菌的好氧特性与产甲烷古菌的厌氧特性合理地解释。网络图分析表明, 丹江口水库上游氨氧化古菌与产甲烷古菌具有显著的相关关系。受丹江口水库运行的影响, 大坝下游水体及沉积物中氨氧化古菌丰度皆比大坝上游少, 而沉积物中产甲烷古菌丰度较高, 二者间相关性不明显。