Abstract: The bacterioplankton community structure in Wujin River was investigated by terminal restriction fragment length polymorphism (T-RFLP) technique in combination with 16S rRNA clone library analysis. The T-RFLP fingerprints analysis shows high diversity and low evenness among the bacterioplankton community. A 16S rRNA gene clone library was also constructed. The results based on Ribosomal Database Project Ⅱ show that there were relatively abundant bacterial species in Wujin River and the predominant bacteria belonged to the genera of Proteobacteria. The BLAST results indicate the proportion of clones with middle or high similarity to known sequences in GenBank was high, while many unknown microorganisms were also present. Moreover, the possible environmental links of known genera were also discussed.

Key words: bacterioplankton community structure, terminal restriction fragment length polymorphism (T-RFLP), clone library , Wujin River

摘要： 通过 T-RFLP 分析和构建 16S rRNA小型基因文库相结合的方法, 研究了江苏省常州市武进港塘桥段微生物群落结构特点。T-RFLP 分析结果表明, 该群落的多样性较高,但均匀度较低。基于 Ribosomal Database Project Ⅱ的分析结果表明, 该处水样的浮游微生物群落种类较多, 还含有尚未分出门的微生物种群, 而变形菌门为最优势的类群。通过与 GenBank 中已知序列对比(BLAST)发现, 具有较高相似性的克隆数较多, 但也存在很多未知种类的新细菌。此外,还探讨了一些已知属的微生物的环境意义。

关键词: 浮游微生物群落, T-RFLP, 克隆文库, 武进港