Abstract: A sludge population optimization strategy aims to select the ammonia oxidation bacteria (AOB) by using sodium chloride (NaCl) as a selective inhibitor to nitrite oxidation bacteria (NOB) is applied in biological nitrogen removal process during municipal wastewater treatment using a sequencing batch reactor( SBR) . Different salinity tests contributed to the best inhibition salinity chosen were applied. In order to optimize nitrifying microbial communities to get steady nitrite pathway, 7.6g/ L salinity and 4 month salt inhibition period were applied. FISH analysis indicated that AOB ( Nitrosospira) became the dominant nitrifying bacteria and NOB ( Nitrobacter) had been washed out of the activated sludge. An understanding of salt inhibition mechanism on NOB is also discussed.

Key words: nitrifying population optimization, salt inhibition, nitrogen removal via nitrite, municipal saline wastewater, inhibition mechanism

摘要： 为了实现稳定的短程硝化, 通过使用 NaCl 作为一种选择抑制剂(只抑制亚硝酸氧化菌(NOB)的生长而不会以抑制氨氧化菌(AOB) 的生长) 在序批式反应器处理含盐生活污水过程中实现硝化种群的优化。实验考察了不同盐度对 AOB 和 NOB 的抑制程度以及对系统硝化性能的影响, 选择 7. 6 g/ L的盐度作为种群优化的最佳盐度。长期抑制实验实施 4 个月后, 亚硝酸盐积累稳定在 95% 以上, 短程硝化稳定。利用荧光原位杂交技术(FISH) 检测到AOB (Nitrosospira) 已经成为硝化菌群的主导菌种, NOB(Nitrobacter)基本检测不出, 证明 NOB 已经被淘洗出系统,硝化种群得到优化。同时讨论了盐度对 NOB 的选择抑制机理。

关键词: 硝化种群优化, 盐度抑制, 短程硝化, 含盐生活污水, 抑制机理