Abstract: The physiology effect of the variation of the uniform dissolved oxidation (DO) on suspension culture of axus cuspidate was studied. The results showed that the cell respiratory was suppressed under the conditions of pure dissolved oxidation (DO=350) and low dissolved oxidation (DO=0 and 10), resulting in the decrease of the mitochondria activity. The contents of the intracellular and extracellular soluble proteins changed little under the low dissolved oxidation culture condition. However, under DO=350 and 40, the intracellular protein tended to increase. In addition, the content of extracellular phenol was accumulated in both pure oxidation and low oxidation conditions.

Key words: dissolved oxidation concentration, Taxus cuspidate, cell culture, mitochondria activity, enzyme activity, soluble protein

摘要： 研究了均匀溶氧体积浓度变化对东北红豆杉悬浮植物细胞的生理影响，结果表明纯氧（DO=350）和缺氧（DO=0和10）培养条件均抑制了细胞的呼吸，导致了细胞线粒体活性降低。缺氧培养条件下细胞内外可溶性蛋白含量变化不大，而在纯氧（DO=350）和DO=40的培养条件下，胞内可溶性蛋白呈现增高趋势。此外，纯氧培养和缺氧培养条件均导致了细胞外酚的积累。

关键词: 溶氧浓度, 东北红豆杉, 细胞培养, 线粒体活性, 酶活, 可溶性蛋白