Acta Scientiarum Naturalium Universitatis Pekinensis

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Comparison of Structures between Crystals of Bar-headed Goose Hemoglobin Grown in Space and Earth

LIANG Yuhe,ZHA Zheng,HUA Ziqian, LIANG Xue,XU Qian,LU Guangying   

  1. National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, 100871
  • Received:2000-04-28 Online:2001-07-20 Published:2001-07-20



  1. 北京大学蛋白质及植物基因工程国家重点实验室,北京,100871

Abstract: During the experiment of protein crystal growth in space carried by Chinese re-entry capsule FSW-2, the crystals of Bar-headed Goose hemoglobin suitable for X-ray analysis were obtained both in space and earth as contrast group. The diffraction data of both crystals were collected, and the structures were solved using molecule replacement methods. The comparison of structures in space and earth indicate that, (1) The interactions among molecules in crystal and subunits of molecule in space is weakened relative to the crystal on earth, (2) There is a contact in the α1β2“switch region” of molecule in space, between α1 Thr41CG2 and β2 His97ND1, which doesn't appear in earth.

Key words: crystal growth in space, bar-headed goose hemoglobin, crystal structure

摘要: 在1994年我国返回式卫星FSW-2上进行的空间蛋白质晶体生长实验中,获得了适合于X射线分析的空间实验组和地面对照组的斑头雁血红蛋白晶体,并收集了X射线衍射数据。应用分子置换法解析了结构,并进行了比较研究。结果显示空间晶体的分子间和分子中亚基间的相互作用趋于减弱,在α1β2“开关区”比地面晶体多一个α1 Thr41CG2与β2 His97ND1的接触。

关键词: 空间晶体生长, 斑头雁血红蛋白, 晶体结构

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