Acta Scientiarum Naturalium Universitatis Pekinensis

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The Foundation of ChelexR100 Method to Extract the Genome of Drosophila Non-Fresh Materials

MA Yunmei, LU Jing, DAI Zhuohua   

  1. College of Life Sciences, Peking University, Beijing, 100871
  • Received:1997-08-16 Online:1998-11-20 Published:1998-11-20


马云梅, 吕静, 戴灼华   

  1. 北京大学生命科学学院,北京,100871

Abstract: The genome of Drosophila immigrans non-fresh materials (soaked in 70% & 100% ethanoland dried materials) was extracted with ChelexR100. Then the ITS1 fragment which is between 18S rRNA and 5.8S rRNA was amplified with primers designed by our laboratory. The amplified fragment was linked to pGEM-T easy Vector (Promega company) and the final plasmid was transformed to E.coli DH5a. Then the plasmid was extracted and digested withendonuclease. According to the result of one of the non-fresh materials, the effects that templates were prepared using the Chelex method and the usual method were the same. Furthermore, the pollution and DNA waste were reduced in the Chelex method.

Key words: Drosophila immgrans, non-fresh materials, rRNA gene, ITS1

关键词: 果蝇, 非新鲜标本, rRNA基因, ITS1

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