Abstract: The monomer of SuMA or SuMA and HEMA was grafted onto the PET fibers successfully under UV irradiation by the use of NaIO₄ instead of the degassing process. Under the same concentration of SuMA, the use of HEMA could enhance the grafting yield of SuMA onto PET fibers obviously comparing with the process which only SuMA was used. As expected, proteins of trypsin and hepatitis surface antibody were immobilized onto the SuMA grafted PET fibers successfully under the neutral aqueous solutions, which was realized through the reaction between the active ester group of SuMA and amino group of protein. Although the use of HEMA during UV grafting could improve the grafting yield of SuMA onto PET fibers, the special impede on the couple reaction of SuMA and proteins caused by the grafted HEMA on PET remarkably inhibited the immobilization of protein, made the amount of immobilized protein lower than that of the only SuMA grafted samples.

Key words: SuMA, UV grafting, trypsin, hepatitis surface antibody, immobilization

摘要： 通过使用NaIO₄而不需除氧的条件下，利用紫外光接枝技术，成功地将SuMA或SuMA和HEMA二元单体共接枝到PET纤维上。在相同SuMA单体浓度下，HEMA的加入能显著提高SuMA的接枝率。如所期望的那样，接枝在PET纤维上的SuMA，在近中性的水溶液中，通过其活性酯基与蛋白质的—NH₂反应，成功实现了对胰蛋白酶和肝炎表面抗体的固定化；HEMA的加入虽然提高了SuMA的接枝率，但是HEMA接枝产生的空阻却显著减少了固定的蛋白质量，使其低于仅SuMA接枝的情况。

关键词: SuMA, 紫外光接枝, 胰蛋白酶, 肝炎表面抗体, 固定化