Acta Scientiarum Naturalium Universitatis Pekinensis

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Analysis of Volatile Components in Ox Feces by Capillary Gas Chromatography

DING Qiang, DENG Yue, SUN Yufang, HUANG Aijin, SUN Yiliang   

  1. College of Chemistry and Molecular Engineniry, Peking University, Beijing, 100871
  • Received:1997-07-24 Online:1998-11-20 Published:1998-11-20


丁强, 邓越, 孙玉芳, 黄爱今, 孙亦梁   

  1. 北京大学化学与分子工程学院,北京,100871

Abstract: According to the traditional Chinese medicine, it was reported that the volatiles of the ox feces are effective in curing many illness. We collected the volatiles of ox feces by using a simultaneous distillation and extraction (SDE) equipment. The chemical composition of the enriched volatiles was examined by means of capillary gas chromatography and combined gas chromatography-mass spectrometry. 50 constituents of the volatiles were preliminarily identified by GC-MS, which amounted to 70.92% of the total peak areas. Of these compounds, 24 were further verified by measuring their temperature-programmed retention indexes or retention times on SE-54 and PEG-20M columns and making comparison with those of the corresponding authentic samples, which amounted to 34.68% of the total peak areas. Another 8 constituents of the volatiles were verified by making comparison with the data in The Sadtler Standard Gas Chromatography Retention Index Library, which amounted to 6.09% of the total peak areas. The main constituents of the volatiles are hexanal (6.20%), dimethyl trisulfide (5.48%), dimethyl disulfide (4.55%), nonanal (4.35%), dimethyl tetrasulphide (3.96%), octanal (3.61%), heptanal (3.22%). The compound classes consist of aldehydes (14), alcohols (3), ketones (12) and acids (2).

Key words: gas chromatography-mass spectrometry, ox feces, volatiles, temperature-programmed retention indexes

摘要: 利用同时蒸馏萃取法(SDE)提取了有治疗恶疮、霍乱效果的牛粪挥发性成分。用毛细管GC-MS联用法分离并初步鉴定了50种组分,占总峰面积的70.92%,进一步用程序升温保留指数和标准样品联合确证了其中的24种化合物,占总峰面积的34.68%,与Sadtler保留指数库文献数据对照又确证了另外8种化合物,占总峰面积的6.09%。其中含量较高的组分是己醛(6.20%),三硫化二甲基(5.48%),二硫化二甲基(4.55%),壬醛(4.35%),二甲基四磺酸酐(3.96%),辛醛(3.61%),庚醛(3.22%)。从化合物的种类来看,主要为:醛类(14个),醇类(3个),酮类(12个),酸类(2个),它们共占总峰面积的44.54%。

关键词: 气相色谱-质谱联用仪, 牛粪, 挥发性成分, 程序升温保留指数定性

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