Abstract: The CZE and HPLC separation of substituted piperazine designed and synthesized by our research group was investigated. In CZE the influence of pH value of the buffer, the source of the capillary, the type and concentration of the buffer was examined. The complete separation of I and III was not obtained becauseof their similar electrophoretic migration velocity under the optimized conditions of CZE. In HPLC the complete separation of the four compounds was obtained employing 85% acetonitrile-15% water as the mobile phase. It demonstrated the two separation techniques based on different separation mechanism may be complementary each other for the separation of drugs.

Key words: substituted piperazines, capillary zone electrophoresis, high-performance liquid chromatography

摘要： 对自行设计合成的哌嗪类药物进行了毛细管区带电泳(CZE)和高压液相色谱(HPLC)的分离研究。在CZE的分离过程中，考察了缓冲液的pH值、毛细管柱来源、缓冲液的类型和浓度、添加剂等因素对分离效果的影响。在优化的条件下，化合物Ⅰ与化合物Ⅲ的电泳迁移率相近，没有得到完全的分离。利用反相HPLC以85%乙腈-15%水作为流动相，4个化合物得到了完全的分离，说明了这两种基于不同分离机制的分析手段在药物分离中可以互为补充。

关键词: 哌嗪类药物, 毛细管区带电泳, 高压液相色谱