Acta Scientiarum Naturalium Universitatis Pekinensis

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Surface Display and Structural Libraries of Peptides

ZHU Shenggeng   

  1. College of Life Sciences, Peking University, Beijing, 100871
  • Received:1996-12-10 Online:1997-07-20 Published:1997-07-20



  1. 北京大学生命科学学院,北京,100871

Abstract: Surface display is a newly developed gene manipulation technology by which fragments of foreign peptides are expressed as fusion proteins displayed on the phage or cell surface, keeping their spatial structure and biological activity relatively independent. This technology may be used for studying the properties, mutual recognition and interaction of peptides (proteins), so as to survey very large display libraries for target functional peptides. There are three display systems, including filamentous bacteriophage, T4 and T7 phage, and cell surface systems, mosthy used so far. Display libraries include recombinant phage antibody libraries, random short peptide libraries, peptide comformation libraries, cDNA display libraries, and gene mutant display libraries. The surface display technology can be used for the production of artificial antibody and vaccine, the determination of antigen epitopes and protein interactive sites, the isolation of regulatory molecules, the study of cell surface engineering, the development of peptide drugs, and the experimentally directed evolution of biomelecules.

Key words: protein transformation, surface display, structural library, target functional peptide, affinity selection, surface display, structural library, target functional peptide, affinity selection, protein transformation

摘要: 表面展示是一种新的基因操作技术,它使表达的多肽以融合蛋白形式展现在噬菌体或细胞表面,保持相对独立的空间结构和生物活性。该技术可用于研究多肽(蛋白质)的性质、相互识别和作用,并据此从巨大展示库中选择特定靶功能的多肽结构。常用丝状噬菌体、T4噬菌体、λ噬菌体以及细胞构建表面展示系统。表面展示库包括重组噬菌体抗体库、随机短肽库、多肽构象库、cDNA展示库和基因突变体展示库。表面展示技术可用于人工抗体和疫苗的制备、抗原决定簇的定位、蛋白质相互作用位点的确定、特异调节分子的分离、细胞表面工程的研究、多肽药物的研制,以及生物分子实验定向进化等研究。

关键词: 表面展示, 结构库, 靶功能多肽, 亲和选择, 蛋白质的改造, 表面展示, 结构库, 靶功能多肽, 亲和选择, 蛋白质的改造

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