Production of MC15-Kunming Chimeras by Culture

Acta Scientiarum Naturalium Universitatis Pekinensis

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Production of MC15-Kunming Chimeras by Culture

HE Wei, WU Heling

College of Life Sciences, Peking University, Beijing, 100871

Received:1996-09-23 Online:1997-03-20 Published:1997-03-20

共培养法获得MC15昆明嵌合鼠

何维, 吴鹤龄

北京大学生命科学学院，北京，100871

Abstract

Abstract: Two MC15-KunMing chimeras were produced by a new method that involves the simple coculture of eight-cell embryos on a lawn of ES cells. Compared with microinjection blastocyst, this methodis feasible.

Key words: ES cell, eight-cell embryo, coculture, chimeras

摘要： 嵌合鼠的获得是通过ES细胞途径得到转基因小鼠的关键环节。以MC15为供ES细胞，用昆明白品系小鼠8细胞期的胚胎为受体胚胎，采用共培养方法我们获得了2只毛色嵌合鼠。通过与囊胚显微注射法比较，表明这种方法是方便可行的。

关键词: ES细胞, 8细胞胚, 共培养, 嵌合鼠

CLC Number:

Q91.21

HE Wei,WU Heling. Production of MC15-Kunming Chimeras by Culture[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

何维, 吴鹤龄 . 共培养法获得MC15昆明嵌合鼠[J]. 北京大学学报（自然科学版）.

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URL: https://xbna.pku.edu.cn/EN/

https://xbna.pku.edu.cn/EN/Y1997/V33/I2/181

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Production of MC15-Kunming Chimeras by Culture

共培养法获得MC15昆明嵌合鼠

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