Acta Scientiarum Naturalium Universitatis Pekinensis

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Some Isozyme Changes Related to Organogenesiss of Bulblets from Bulb Scales of Lilium davidii in vitro

ZHOU Yibing, FENG Yun, YOU Ruilin   

  1. College of Life Sciences, Peking University, Beijing, 100871
  • Received:2019-09-06 Online:1995-07-20 Published:1995-07-20



  1. 北京大学生命科学学院,北京,100871

Abstract: The bulb scales of Lilium were used as explants and cultured on MS basic medium supplemented with 1 mg/L IBA and 0.5 mg/L 6-BA to build up an experimental system for studying organogenesis in vitro. At about the 10th day of culturing, bulblets grew out from adaxial surfaces of the bulb-scales. The induction rate was 100%. Specimens were taken everyday since the 1st day of culturing for cytohistological observations and the extractions of proteins. The extracts analysed by isoelectrofocusing polyacrylamide gel electrophoresis (IEF-PAGE). The results were as follows: The amount of total protein kept stable in the first two days and decreased to the lowest at the fifth day of culture, and then increased, but decreased again from the seventh day. There were changes of proteins with pI between 6.2 and 6.6. Both the composition and the amount of each component changed. During the culture of Lilium bulb scales in vitro, both the activity of peroxidase isozymes and the kinds of peroxidase isozymes increased gradually. Four distinct bands appeared in the cathode zone. The amounts of esterase isozymes with pI4.6~5.9 and 6.6~8.2increased. The amount and the activity of amylase isozymes also increased gradually. These results suggested that a further investigation using this system could lead to a better understanding of the isozyme changes in organogenesis.

Key words: bulb scales of Lilium davidii, organogenesis, isozyme

摘要: 本研究利用组织培养技术,在附加1mg/L IBA和0.5mg/L 6-BA的MS培养基上建立起兰州百会鳞片外植体体外器官发生实验系统。培养10天左右,外植体上就可以诱导出小鳞茎,发生率为100%。从接种之日起每天取材固定作细胞学观察和提取水溶性蛋白质作聚丙稀酰胺凝胶等电聚焦电泳(IEF-PAGE)分析,结果表明离体培养过程中,前2天外植体内蛋白质含量基本稳定,2天后含量降低,第5天降至最低点后,含量又上升,第7天后又开始降低。蛋白质变化主要发生在pI6.2~8.2间,既有蛋白质组成成分的变化,又有各组成成分含量的变化。在百合鳞片离体培养过程中,过氧化物酶活性逐渐增强,过氧化物酶同工酶数量增多,在阴极端出现四条区别明显的条带。酯酶同工酶数量增多,变化发生在pI4.6-5.9 及 pI6.6-8.2间,淀粉酶同工酶数量逐渐增多和活力逐渐增强。

关键词: 百合鳞片, 离体形态发生, 同工酶

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