DNA Ioslation and Sequence Analysis of Dinosaur DNA from Cretaceous Dinosaur Egg in Xixia Henan, China

Acta Scientiarum Naturalium Universitatis Pekinensis

DNA Ioslation and Sequence Analysis of Dinosaur DNA from Cretaceous Dinosaur Egg in Xixia Henan, China

LI Yi, AN Chengcai, ZHU Yuxian, ZHANG Yun, LIU Yifei, QU Lin, YOU Lingtao, LIANG Xiaowen, LI Xiaohua, QU Lijia, ZHOU Zengquan, CHEN Zhangliang

College of Life Sciences, Peking University, Beijing, 100871

Received:1995-02-05 Online:1995-03-20 Published:1995-03-20

中国河南西峡晚白垩世恐龙蛋化石基因分离及序列分析

李毅,安成才,朱玉贤,张昀,刘一飞,曲林,由凌涛,梁晓文,李小华,瞿礼嘉,周曾铨,陈章良

北京大学生命科学学院，北京，100871

Abstract

Abstract: DNA was extracted from 70-80 million year old dinosaur egg excavated from Cretaceous belt formation in Xixia Henan, China. The DNA samples from both outer shell and inner floccule contents were used as templates in polymerase chain reactions (PCR) with a specific primer andhexamers. With these two primers a 150bp DNA fragment has been amplified, cloned and sequenced. Deduced amino acid sequence of this dinosaur DNA hasbeen compared with known protein sequences in EMBL Bank. The results showedthat it has significant homology with Xenopus laevis epithelia precuraor-EP-cadherin (40.6%) and brain-cadherin (37.5%), bovine placental cadherin (34.4%) and human placental cadherin (36.4%), respectively.

Key words: dinosaur DNA, cadherin, Xixia

摘要： 从河南省西峡晚白垩世一枚恐龙蛋化石内腔絮状内含物及外壳提取DNA样品，并用一段特异引物与6碱基随机引物进行PCR扩增、基因克隆和序列分析。结果表明用这对引物从蛋腔絮状内含物样品中扩增到一段150bp的DNA片段，推测氨基酸序列与EMBL库中已知蛋白质氨基酸序列进行了序列同源性分析，结果表明所克隆到的这段基因与非洲爪蟾表皮钙粘着蛋白(cad-herin)前体及脑细胞粘着蛋白、牛及人胎盘胞钙粘着蛋白在氨基酸水平有显著的序列同源性，分别为40.6%，37.5%，34.4%和36.4%。

关键词: 恐龙DNA, 钙粘着蛋白, 西峡

CLC Number:

Q915.21

LI Yi,AN Chengcai,ZHU Yuxian,ZHANG Yun,LIU Yifei,QU Lin,YOU Lingtao,LIANG Xiaowen,LI Xiaohua,QU Lijia,ZHOU Zengquan,CHEN Zhangliang. DNA Ioslation and Sequence Analysis of Dinosaur DNA from Cretaceous Dinosaur Egg in Xixia Henan, China[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

李毅,安成才,朱玉贤,张昀,刘一飞,曲林,由凌涛,梁晓文,李小华,瞿礼嘉,周曾铨,陈章良. 中国河南西峡晚白垩世恐龙蛋化石基因分离及序列分析[J]. 北京大学学报（自然科学版）.

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URL: https://xbna.pku.edu.cn/EN/

https://xbna.pku.edu.cn/EN/Y1995/V31/I2/148

[1]	AN Chengcai,LI Yi,ZHU Yuxian,SHEN Xing,ZHANG Yun,YOU Lingtao,LIANG Xiaowen,LI Xiaohua,WU Si,WU Peng,GU Hongya,ZHOU Zengquan,CHEN Zhanglian. Molecular Cloning and Sequencing the 18S rDNA from Specialized Dinosaur Egg Fossil Found in Xixia Henan, China [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1995, 31(2): 140-147.
[2]	ZHANG Yun,FANG Xiaosi. A Late Cretaceous Dinosaur Egg with Preserved Genetic Information from Xixia Basin, Henan, China: Structure, Mineral-Chemical and Taphonomical Analyses [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1995, 31(2): 129-139.