Metabolic Influence of Mercury Contamination on Bacterial Mercury Methylation by Geobacter sulfurreducens PCA

doi:10.13209/j.0479-8023.2022.036

Acta Scientiarum Naturalium Universitatis Pekinensis ›› 2022, Vol. 58 ›› Issue (3): 526-536.DOI: 10.13209/j.0479-8023.2022.036

Previous Articles Next Articles

Metabolic Influence of Mercury Contamination on Bacterial Mercury Methylation by Geobacter sulfurreducens PCA

TAO Huchun^†, QIAN Lu, ZHANG Lijuan, DING Lingyun, FANG Fang, DAI Maifan, WEI Ruqian

Shenzhen Key Laboratory for Heavy Metal Remediation and Reclamation, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055

Received:2021-05-14 Revised:2021-06-11 Online:2022-05-20 Published:2022-05-20
Contact: TAO Huchun, E-mail: taohc(at)pkusz.edu.cn

汞胁迫对Geobacter sulfurreducens PCA汞甲基化作用的代谢组影响

陶虎春^†, 钱璐, 张丽娟, 丁凌云, 方芳, 戴迈凡, 魏如倩

北京大学深圳研究生院环境与能源学院, 深圳市重金属污染控制与资源化重点实验室, 深圳 518055

通讯作者: 陶虎春, E-mail: taohc(at)pkusz.edu.cn
基金资助:
国家自然科学基金(51679002, 51809004)和深圳市基础研究项目(JCYJ20180503182122539)资助

Abstract

Abstract:

Metabolomics, a rising omics approach, was employed to screen out cellular significantly differential metabolites and analyze related metabolic pathways in bacterial Hg-methylating process. Under the stress of Hg(II) at environmentally relevant concentrations (0?100 μg/L), the adsorbed/assimilated Hg(II) was utilized by Hg-methylator of Geobacter sulfurreducens PCA to reduce and methylate Hg(II). The highest efficiency of 3.09%± 0.16% was achieved for bacterial Hg-methylation upon exposure to 10 μg/L Hg(II). Metabolomics data showed that carbohydrate metabolism, amino acid metabolism and nucleotide metabolism were disturbed by Hg(II) stress in the PCA cells. To withstand the stress of Hg(II), G. sulfurreducens PCA increased energy demand for Hg methylation and DNA repair.

Key words: mercury, Geobacter sulfurreducens PCA, methylation, metabolomics

摘要：

采用新兴的代谢组学技术, 筛选差异性细胞代谢物, 分析甲基汞生成和调控的关键代谢通路。在典型环境污染浓度 (0~100 μg/L) Hg(II)的胁迫下, 汞甲基化微生物Geobacter sulfurreducens PCA吸附/吸收的Hg(II)成为参与汞还原和甲基化的主要反应物质。初始 Hg(II)浓度为10 μg/L时, G. sulfurreducens PCA达到最高汞甲基化效率3.09%±0.16%。代谢组学数据显示, Hg(II)胁迫对胞内的碳水化合物代谢、核苷酸代谢和氨基酸代谢造成干扰。为了应对Hg(II)胁迫, G. sulfurreducens PCA增大了对能量的需求, 用来调控汞生物甲基化反应, 并对受损DNA进行修复。

关键词: 汞, Geobacter sulfurreducens PCA, 甲基化, 代谢组学

TAO Huchun, QIAN Lu, ZHANG Lijuan, DING Lingyun, FANG Fang, DAI Maifan, WEI Ruqian. Metabolic Influence of Mercury Contamination on Bacterial Mercury Methylation by Geobacter sulfurreducens PCA[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2022, 58(3): 526-536.

陶虎春, 钱璐, 张丽娟, 丁凌云, 方芳, 戴迈凡, 魏如倩 . 汞胁迫对Geobacter sulfurreducens PCA汞甲基化作用的代谢组影响[J]. 北京大学学报自然科学版, 2022, 58(3): 526-536.

Add to citation manager EndNote|Ris|BibTeX

URL: https://xbna.pku.edu.cn/EN/10.13209/j.0479-8023.2022.036

https://xbna.pku.edu.cn/EN/Y2022/V58/I3/526

[1]	WANG Xisong, SHI Gang, DING Lingyun, ZHANG Yaoyu, ZHANG Shanfa, Maurycy Daroch, TAO Huchun, ZHANG Lijuan. Pathways and Mechanisms of Nitrogen Removal by Hydrogen-Oxidizing Bacteria under Stress of Quinolone Antibiotics [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2024, 60(3): 539-547.
[2]	PENG Mou, LI Jianghai, YANG Bo. Pore Structure Characteristics and Influencing Factors of Deep Sandstone Reservoirs: A Case Study of Guaizihu Depression in Yin’e Basin [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2024, 60(2): 249-264.
[3]	PANG Shan, MEI Qiliang, ZHANG Hongjun, WANG He, SUN Tong, GUAN Ping, SHI Yongmin. Section and Rate-controlled Mercury Injection and Its Differential Mechanisms: An Example of Chang-2 Reservoir of Yanchang Formation in a Block of Ordos Basin [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2019, 55(5): 907-914.
[4]	TAO Huchun, YANG Sai, DING Lingyun, ZHANG Lijuan, HE Ningning, ZHANG Yaoyu. Study on the Effect of Two Red-Tide Algae on Mercury Biocondensation and Methylation [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2019, 55(4): 709-716.
[5]	XIE Tianning, LIN Yan, QIU Xinghua. A Preliminary Metabolomics Study on Air Pollution Associated Acute Biomarkers of Health Effect Based on a Migration Panel between Los Angeles and Beijing [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2019, 55(3): 501-508.
[6]	TAO Huchun, MA Biao, DING Lingyun, WU Qingqing, GAO Yue. Influence of Two Quinolone Antibiotics on Mercury Methylation Bacteria of Geobacter sulphurreducens PCA [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2018, 54(5): 1039-1045.
[7]	CHEN Zhijun,ZHANG Yinsong,ZENG Limin,WEI Yongjie,TANG Xiaoyan. Application and Problem Discussion of On-line Atmospheric Mercury Analyzer in Field Measurement: Take Measurement in Wuxi in Winter for Example [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2012, 48(4): 665-669.
[8]	YU Zhongying,ZENG Limin,ZHANG Yuanhang. Design and Application of On-line Atmospheric Mercury Analyzer [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2006, 42(6): 757-761.
[9]	ZHOU Lingping,DU Daming,LI Xuanwen,WANG Shaokun,ZHOU Aiqiu,LIU Jian. Studies on the Catalytic Behaviors of NaBr/KZSM-5 Catalyst for the Oxidative Methylation of Toluene with Methane [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2002, 38(2): 153-158.

Metabolic Influence of Mercury Contamination on Bacterial Mercury Methylation by Geobacter sulfurreducens PCA

汞胁迫对Geobacter sulfurreducens PCA汞甲基化作用的代谢组影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 9

Recommended Articles

Metrics