Abstract: Reffering to mitochondrial genome of Silurus glanis, the authors designed 6 pairs of primers for the long and accurate PCR amplification of a Yellow River catfish (S. lanzhouernsis). Sequence analysis indicated that the mitochondrial genome of S. lanzhouensis has 16524 bp necleitides, comprising 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a non-coding control region. The phylogenetic analysis of 11 species in Siluriformes was conducted with maximum likelihood method based on nucleotide sequence of all protein-coding genes. The result shows that S. lanzhouensis had close phylogenetic relationship with other species in Siluridae.

Key words: Silurus lanzhouensis, mitochondrial genome, phylogenetics

摘要： 利用GenBank中欧洲鲇的线粒体基因组序列, 设计出6对引物, 通过PCR扩增产物直接测序和引物行走(primer walking)法测定了兰州鲇的线粒体基因组序列, 并对其进行结构分析。兰州鲇线粒体基因组序列全长16524 bp, 包括37个基因(2个rRNA基因、22个tRNA基因和13个蛋白质编码基因)和1个非编码区。用最大似然法对鲇形目11种鲇鱼线粒体基因组的13个蛋白质编码基因的核苷酸序列进行系统发育分析。结果显示, 兰州鲇首先跟其他鲇科鱼类聚为一支, 形成一个单系群, 位于系统发育关系树的中部。

关键词: 兰州鲇, 线粒体基因组, 系统发生