Crystallization and Preliminary X-ray Studies of Metallothionein-II from Rabbit Liver

Acta Scientiarum Naturalium Universitatis Pekinensis

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Crystallization and Preliminary X-ray Studies of Metallothionein-II from Rabbit Liver

AN Yu¹, LI Genpei², RU Binggen¹

¹College of Life Sciences, Peking University, Beijing, 100871; ²College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871

Received:1999-01-14 Online:2000-03-20 Published:2000-03-20

兔肝金属硫蛋白-Ⅱ的晶体培养及初步衍射研究

安钰¹,李根培²,茹炳根¹

¹北京大学生命科学学院，北京，100871；²北京大学化学与分子工程学院，北京，100871

Abstract

Abstract: Metallothionein-II, 6.8kDa, purified from the liver of rabbit induced by cadmium, has been crystallized in the space group P6₃22 using hanging drop vapor diffusion method, with unit cell parameters a=b=11.34nm, c=14.66nm. There are 8 molecules per asymmetric unit and a solvent content of 58%.

Key words: metallothionein, hanging drop vapor diffusion method, space group, unit cell parameters, asymmetric unit

关键词: 金属硫蛋白, 悬滴汽相扩散法, 空间群, 晶胞参数, 不对称单位

CLC Number:

O743.52

AN Yu,LI Genpei,RU Binggen. Crystallization and Preliminary X-ray Studies of Metallothionein-II from Rabbit Liver[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

安钰,李根培,茹炳根. 兔肝金属硫蛋白-Ⅱ的晶体培养及初步衍射研究[J]. 北京大学学报（自然科学版）.

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Crystallization and Preliminary X-ray Studies of Metallothionein-II from Rabbit Liver

兔肝金属硫蛋白-Ⅱ的晶体培养及初步衍射研究

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