Acta Scientiarum Naturalium Universitatis Pekinensis

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Identification and Expression of the Product from Engineering Bacterium Containing Recombinant Human Interleukin-18(IL-18) Gene

DONG Yuqing, LI Ronggui, DONG Chunxia, ZHAO Jindong, WU Guangyao   

  1. College of Life Sciences, Peking University, Beijing, 100871
  • Received:1999-03-04 Online:2000-03-20 Published:2000-03-20


董宇清, 李荣贵, 董春霞, 赵进东, 吴光耀   

  1. 北京大学生命科学学院,北京,100871

Abstract: The cDNA of human Interleukin-18 was obtained by RT-PCR from the mRNA of human peripheral blood cells. The DNA sequencing was performed and the DNA was cloned into expression vector pBV220. The overexpression was performed in E.coli HB101. The expressive product was purified through Sephadex G100 and identified by N-terminal amino acid sequencing and Western blot.

Key words: human interleukin-18 (hIL-18), gene cloning, expression

关键词: 人白细胞介素18, 基因克隆, 表达

CLC Number: