Abstract: The way to obtain the prolonged donor-specific tolerance after skin allograft must include both the specific lysis of the immunocompetent cells in the peripheral blood and lymphoid, and the induction of the recognition as self-antigen in the central immunological organ. The adult BALB/C mouse recipient was tail-vein injected with C₅₇BL/6 donor splenocytes loaded ricin, a kind of plant toxin. The guided toxic cells selectively killed the T, Blymphocytes which could specifically recognize the allografted antigens on the surface of C₅₇BL/6 cells. In advance, the recipient had been given C₅₇BL/6 splenocytes via the intrathymic route to achieve clone deletion of alloreactive T cells during development. These treatments induced a donorspecific tolerance which allowed the skin grafts on the BALB/C mice to survive prolongedly without the need for further immunosuppression (mean survival time[MST]>60 days, control group's MST=5.0 days). The results of MTT colorimetric assay after one-way mixed lymphocytes co-culture provided the cellular evidence in vitro.

Key words: immunity to transplantation, donor-specific tolerance, donor cell intrathymic injection, T cell cloned deletion, ricin directed lysis

摘要： 通过2个不同品系小鼠间进行皮肤移植，研究免疫系统产生特异耐受的机制。要获得对异体抗原的特异性耐受，必须包括对外周淋巴器官中免疫效应细胞的特异杀伤，并同时诱导中枢免疫器官将其作为“自我”物质进行识别，导致系列克隆流产。以BALB/C鼠为受体，以C₅₇BL/6(简称C₅₇)鼠为供体，用包裹蓖麻毒素蛋白(ricin)的供体淋巴细胞作为“导弹毒剂”，对受体产生的排异供体移植抗原的T,B淋巴细胞进行定向杀伤，并事先用供体淋巴细胞对受体进行胸腺内注射，以使得识别移植抗原的T淋巴细胞的克隆流产。实验组移植皮片平均存活超过60d(天)(对照组为5d), t检验证明差异极其显著。单向混合淋巴细胞培养的MTT比色分析结果为导向毒细胞的定向杀伤功能和供体淋巴细胞胸腺注射的T细胞克隆衰减功能提供了细胞学证据。

关键词: 移植免疫, 供体特异性免疫耐受, 供体细胞胸腺注射, T细胞克隆流产, 蓖麻毒蛋白定向杀伤