In order to explore the combined toxicity of carbon nanomaterial graphene oxide (GO) and perfluorooctane sulfonate (PFOS) in freshwater benthic shellfish, Corbicula fluminea was used as the target organism to study the effects of single and co-exposures of 1 mg/L GO or/and 500 ng/L PFOS for 28 days on body length, body weight, filtration rate, level of reactive oxygen species (ROS), antioxidant enzyme system and malondialdehyde (MDA) content. The toxicity was evaluated by enhanced integrated biomarker response (EIBR). The results showed that the body length and body weight of C. fluminea did not change significantly after exposure. Compared with the blank control and solvent control groups, the filtration rates in the single and co-exposure groups significantly decreased. Both GO and PFOS stresses significantly changed the enzyme responses in gills and visceral masses of C. fluminea with consistent variation trends in both organs. The EIBR results showed that the toxicity in gills and visceral masses of the co-exposure group was stronger than that of the PFOS or GO single exposure groups. 

为了探索碳纳米材料氧化石墨烯(GO)和全氟辛烷磺酸类物质(PFOS)在淡水底栖贝类体内的联合毒性效应, 以河蚬为研究对象, 考察 1 mg/L GO和500 ng/L PFOS单独及联合暴露28天后对河蚬体长、体重、滤食率、活性氧水平、抗氧化系统酶活性和丙二醛含量的影响, 并采用优化的综合生物标志物响应指数(EIBR)进行整体评估。结果表明, 暴露结束后, 河蚬的体长和体重没有明显的变化。与空白对照组和溶剂对照组相比, GO和PFOS的单独暴露组及联合暴露组的滤食率均显著下降。在河蚬的鳃和内脏团中, GO和PFOS的胁迫都会引起抗氧化系统酶活性响应的显著变化, 且两器官中变化趋势一致。EIBR结果表明, 鳃和内脏团中联合暴露组的毒性比 PFOS或GO单独暴露组的毒性更强。