The effects of BDE-209 (decabrominated diphenylether-209) on the proliferation and apoptosis of human normal liver L-02 cells were analyzed by CCK-8 and Hoechst 33342/PI methods. The results showed that the inhibition rate of cell proliferation increased with the increase of BDE-209 concentration after 24 hours exposure at different concentration levels (0–70 μg/mL). The IC₅₀ of BDE-209 on human normal L-02 cells for 24 hours was 127.08±24.93 μg/mL, which was analyzed by SPSS-Probit-Logit method. After 24 hours exposure to BDE-209 at different concentration levels (0–15 μg/mL), there was no significant change in cell morphology in each dose group compared with the control group, and only cell reduction was observed in the 15 μg/mL dose group. After apoptotic staining, fluorescence microscopy showed that the number of cells stained with brilliant blue and light powder increased with the increase of BDE-209 concentration, and the apoptosis rate of human normal liver L-02 cells increased with the increase of BDE-209 concentration. Compared with the control group, the difference of each dose group was statistically significant. The results showed that BDE-209 could inhibit the proliferation of human normal liver L-02 cells and increase the apoptotic rate.

采用Cell Counting Kit-8 (CCK-8)和Hoechst 33342/PI等, 分析十溴联苯醚(BDE-209)对人正常肝L-02细胞增殖和凋亡的影响。结果显示, 在不同浓度水平(0~70 μg/mL)的BDE-209中暴露24小时后, 细胞增殖抑制率随着BDE-209浓度的升高而升高。经SPSS-Probit-Logit法计算, 得出 BDE-209对人正常肝L-02细胞24小时的IC₅₀为127.08 ±24.93 μg/mL。在不同浓度水平(0~15 μg/mL)的BDE-209中暴露24小时后, 与对照组相比, 各剂量组细胞未出现明显的细胞形态变化, 仅在15 μg/mL剂量组观察到细胞减少的现象。凋亡染色后, 荧光显微镜检发现, 随着BDE-209浓度升高, 亮蓝、淡粉染色细胞数量增大, 人正常肝L-02细胞的凋亡率增高。与对照组相比, 各剂量组差异均具有统计学意义。分析结果表明, BDE-209可引起人正常肝L-02细胞增殖受到抑制, 并诱导细胞凋亡率增加。