Acta Scientiarum Naturalium Universitatis Pekinensis ›› 2017, Vol. 53 ›› Issue (6): 1161-1164.DOI: 10.13209/j.0479-8023.2017.077

• Orginal Article • Previous Articles     Next Articles

Isolation of Fungal Strains in Soil of Selenium Mining Area and Screening for Their Anti-tumor Activity

Xiling RAN1, Yu LIU1, Congkui TIAN1(), Tingting ZHU2, Yanan SONG1, Yuexian MO1   

  1. 1. Key Laboratory of Biological Resources Protection and Utilization of Hubei Province & Wuling Mountain Institute of Natural Medicine, Hubei University for Nationalities, Enshi 445000;
    2. State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology & Shenzhen Academy of Environmental Sciences, Shenzhen 518001;
  • Received:2016-07-22 Revised:2016-09-14 Online:2017-11-20 Published:2017-11-20

硒矿区土壤中真菌的分离培养及抗肿瘤活性筛选

冉喜玲1, 刘雨1, 田从魁1(), 朱婷婷2, 宋雅楠1, 莫月贤1   

  1. 1. 生物资源保护与利用湖北省重点实验室, 湖北民族学院武陵山天然药物研究所, 恩施 445000
    2. 国家环境保护饮用源地管理技术重点实验室, 深圳市环境科学研究院, 深圳 518001
  • 基金资助:
    湖北省高校优势特色学科群建设计划(鄂学位[2015]5)、生物资源保护与利用湖北省重点实验室重点项目(PKLHB1504)、深圳市科技研发资金(JCYJ20130402094730839)、湖北民族学院博士启动基金(4138004)和湖北民族学院大学生创新创业项目(2016CX0715)资助

Abstract:

The fungal strains were obtained by single colony isolation technique from the soil of the world’s unique proved independent selenium deposit. The anti-tumor activity was assayed by MTT method using MDA-MB-231cells, Hela cells and SMMC-7721 cells, respectively, together with morphological observation of these cells under inversed light microscope. The 73 terrestrial fungal strains were isolated from the soil samples collected from 6 different areas in Enshi area of HuBei Province, China. Among them, 32 strains produced fermentation products obviously inhibiting the Hela cells, 13 strains inhibiting the MDA-MB-231 cells and 9 strains inhibiting the SMMC-7721 cells with IC50 value less than 90 µg/ml. These antitumor-metabolite-producing strains have provided fungal strains for further studies on the bioactive metabolites.

Key words: fungi, selenium mining area, terrestrial fungus, isolation and cultivation, anti-tumor activity

摘要:

利用单菌落挑选与划线培养技术, 从硒矿区土壤样品中分离纯化真菌。通过摇床发酵培养, 提取制备发酵样品。采用 MTT 法, 用 MDA-MB-231 细胞、Hela 细胞、SMMC-7721 细胞测试发酵样品的抗肿瘤活性。从采自湖北恩施全球唯一独立硒矿床区的 6 个不同土样中分离真菌73株。其中, 对人宫颈癌Hela细胞的IC50小于90 µg/mL的菌株32株, 对人乳腺癌MDA-MB-231细胞的IC50小于90 µg/mL的菌株13株, 对人肝癌SMMC-7721细胞的IC50小于90 µg/mL的菌株9株。这些活性菌株为筛选抗肿瘤天然产物提供了良好的药源菌株。

关键词: 真菌, 硒矿床区, 陆生真菌, 分离培养, 抗肿瘤活性