Table of Content

20 July 2002, Volume 38 Issue 4

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Trends and Spatial Distributions of Methane Emissions in Beijing

SU Fang,SHAO Min,ZHANG Min

2002, 38(4):  466-473. 

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Based on the methodology guidelines of IPCC, a city-scale methane emission inventory for Beijing in 1999 was established. The statistical data were derived from newly intensive survey and updated emission factors were referenced. Results showed that the total methane emissions were 296.29 Gg. The main sources were municipal solid waste and fossil fuel, which produced 161.60 Gg and 96.08 Gg of methane emissions, accounting for 54.6% and 32.4% of total emissions respectively. The spatial distributions of methane emission source strengths were also obtained.

The Efferent Modulation Effect of AP Neuron on the Positional Information of Pressure Stimulus in the Leech, Whitmania pigra

JIN Wenjun,ZHANG Renji

2002, 38(4):  487-491. 

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The Anterior Pagoda neuron (AP) is one of the largest neurons in the leech nervous system. It receives multiple sensory inputs. The physiological function of AP is unknown. With the combination of intra-and extra-cellular electrophysiological recording methods, it is suggested that AP neuron modulates the membrane potential of multiple muscle cells in the contralateral body wall.

Preparation and Application of Polyclonal Antibody to Butachlor

GUO Yongen,CHEN Jiahua,WANG Nengdong,ZHANG Xiu,GUO Zhenquan,JIN Sheng

2002, 38(4):  447-452. 

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Polyclonal antibody to butachlor was prepared from rabbits immunized with butachlor-BSA conjugate, and a competitive ELISA was developed for the detection of the butachlor (a major component in a herbicide) within the range of 1×10^-9～100×10^-9mg/mL without cross reactions with the structurally similar chloroacetanilide herbicides, alachlor and acetochlor. This method could also be used to monitor the butachlor concentration of water samples from rice field.

Gold Stripping from Loaded Organic Phases by Inorganic Salts

JIANG Jianzhun,ZHOU Weijin,GAO Hongcheng,WU Jinguang,XU Guangxian,CHEN Jing

2002, 38(4):  453-458. 

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Gold(Ⅰ) Stripping from quaternary ammonium (TDMBAC) loaded-organic phase by inorganic salts such as NH₄SCN, NaClO₄ and KI was described by ¹⁹⁸Au radioactive tracer method. The effect of several variables such as stripping agent concentrations, the volume ratio of stripping agents to gold loaded organic phase and the continuous extraction after stripping was studied under 20℃. It is found that NH₄SCN and NaClO₄ are excellent stripping agents for both n-octanol and TBP system. The organic phase can be rebirth by KCl aqueous solution. The stripping efficiency was agreement with perchloride effect. The effect of cosolvent on the stripping percentage was also discussed.

Production and Characterization of Anti-estriol Monoclonal Antibody

WANG Yongcheng,CHANG Wenbao,GUO Zhenquan

2002, 38(4):  459-465. 

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Estriol (E₃) was bound to bovine serum albumin (BSA) to form a complete antigen (E₃-BSA), after the hydroxy (-OH) at the position of C3 was activated. A BALB/c mouse was immunized with the mixture of E₃-BSA and Freund adjuvant. Monoclonal antibody-producing hybridoma anti-E₃ was prepared by fusion of SP2/0 murine myeloma cells with spleen cell isolated from the immunized mouse. A monoclonal antibody (McAb) specific for E₃ was produced in vivo by the hybridoma anti-E₃. Analysis revealed that anti-E₃ McAb (E₃-McAb) was of the IgG1 type,with the molecular weight 168000 u. The titer of E₃-McAb was 6.0×10^-10 mol/L, and the affinity constant of E₃-McAb with coated complete antigen was 4.7×10⁷ L/mol.

A Study on the Yangfan Quaternary Section, Linyi, Shanxi

FU Jianli,LI Youli

2002, 38(4):  517-523. 

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Lithological characteristics, sedimentary structure, mass-specific magnetic susceptibility and frequency-dependent magnetic susceptibility studies and correlation to the δ¹⁸O curve of DSDP607 & V30-97 show that loess began to deposit on the terrace of the Yellow River about 1.20 Ma B P, and passed thirteen climatic changes from drier and cooler to warmer and wetter later. The turn of sedimentation from alluvium to loess, an event of transformation from accumulation to down-cut of the Yellow River, indicates a sharp tectonic uplift occurred around 1.2 Ma B P.

Selection of the Peptides Binding to ICAM-1 from Phage Display Random 15mer Peptide Library

ZHAO Lequn,ZHAO Jiahui,CHENG Qingli,HUANG Yixiu,ZHU Shenggeng

2002, 38(4):  474-477. 

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A phage display 15mer random peptide library expressed on the N-terminal of the filamentous phage M13 minor coat protein pⅢ was constructed using the phagmid pCANTAB5G8. The capacity of library is 1.36×10⁸, calculated from the transformation efficiency. Human recombinant Intercellular Adhesion Molecule 1 was immobilized on the ELISA plates. After 4 rounds affinity panning and ELISA analysis, 5 positive phage clones that can bind to ICAM-1 specifically were selected. These positive clones were sequenced and the amino acid sequence of 15mer were deduced.The Ka of one positive clone was 7.87×10⁷, measured by ELISA.

The Simulation of Mesoproterozoic Tectonic Stress Field Forming Mafic Dyke Swarms in the Central North China Craton

HOU Guiting,QIAN Xianglin,LI Jianghai

2002, 38(4):  493-496. 

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The Mesoproterozoic mafic dyke swarms are extensively distributed in the central North China Craton(NCC), which are not deformed and metamorphic, emplacing the pre-existed fractures, so the dyke swarms become the conspicuous marks to reconstruct the Mesoproterozoic tectonic stress field of the central NCC. The mafic dykes in the northern block are transtensional while the dykes in the central and southern blocks are extensional. The Mesoproterozoic tectonic stress field could be simulated on the analysis of the distribution and mechanical origin of the Mesoproterozoic mafic dyke swarms in the central NCC. The simulation result shows that the mafic dyke swarms were formed in the extensional tectonic setting in the NCC in the Mesoproterozoic time, which are related to the extension of the Mesoproterozoic Yanliao-Zhongtiao aulacogens cross the central NCC.

The Seismic Stratigraphic Analysis of Taowan Conglomerate in Luonan, Shaanxi

GUO Liyu,GAN Zhimao,ZHANG Guowei,DU Yuansheng

2002, 38(4):  497-503. 

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Taowan Conglomerate was formed with underwater seismism at early Cambrian period and consists of Shattering sedimentary rock, Tsunami rock, Seismic turbidite and Seismic volcanic rock. The Shattering sedimentary rock consists of folded rock, cracked rock and autobreccia. The Seismic turbidite is located in the south of Luonan Ductile Shear Zone and is called the narrow Taowan Conglomerate. Contrarily the broad Taowan Conglomerate consists of Shattering sedimentary rock, Tsunami rock located in the north of the Zone. The seismic sequence of Shattering sedimentary rock-Tsunami rock-Seismic turbidite-Seismic volcanic rock-Normal sedimentary rock was first found in Luonan Shanxi and is rare among the reported examples about it, so it is a good example and offers power evidences to study how the southern marginal basin of Huabei plate transferred to Qinling orogenic belt.

A Study of Intensity of the Faults Activity in Jiyang Depression in Mesozoic and Cenozoic

YI Xuelei,HOU Guiting

2002, 38(4):  504-509. 

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Jiyang depression is a field where nonhydrocarbon is rich.These resources have close relation with the activities of the volcanos. At the same time,the activities of volcanos have deep contact with the activities of the faults. The growth of the faults in Jiyang depression is great. By the research of the index number of the faults growth and the other researches, NW direction faults are the most ones in Mesozoic, NW and NE and approximately EW directions all exist in Cenozoic. The above reflects the whole geotectonic background that sinistral shearing stress field exists in Mesozoic, but dextral shearing stress field exists in Cenozoic.

Advance and the Status Quo of the Research on Applications of Stoneley Wave in Well Logging

HE Tao,SHI Ge

2002, 38(4):  510-516. 

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Stoneley wave is a kind of guide waves propagating along the liquid-solid interface in well.When a sonic beam strikes the surface of a thick plate immersed in a liquid, a Stoneley wave is generated. Stoneley wave approach is applied on analysis of well logging more and more widely in recent ten years with the introduction of advanced analysis methods for component waves from full-waveform array acoustic log data. The spectrum of Stoneley waves are used to identify the fractured zones and quantitatively calculate the fracture parameters of reservoirs, especially to evaluate the validity of fractures at fracture-type complex reservoir.

Tectonic Geometric Characters of Kongxi Structural Belt

ZHANG Jun,PAN Mao,QI Jiafu,YANG Panxin

2002, 38(4):  524-530. 

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Kongxi buried hill shows following features in the seismic profiles: the structures are asymmetric. There is flexural syncline on the east side and a monocline on the west side. Asymmetric repetition of stratigraphic units is present in the drill wells and the dips of beds and faults are gentle. The Permo-Carboniferous system of both sides almost lie in the same slope and the events of Cambrian or upper Proterozoic are continuous from east to west. The phenomenas suggest that Kongxi buried hill could be a thrust belt been traced which consists of reverse faults. The imbricate faults mainly effect Pre-Jurassic strata. There are obvious angular unconformity between Jurassic-Cretaceous system and Paleozoic involved deformation of thrust. The thrust front in some place has cut off the beds of Jurassic-Cretaceous system. The structural style of the thrust structure changes along the strike of the Kongxi buried hill.

Pilsenite First Found in China

LIU Yulin

2002, 38(4):  531-534. 

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Pilsenite is a rare mineral and its information is deficient. The first found pilsenite in China is in Gaozhuang, Henan province. Pilsenite, associated with pyrrhotite, pyrite, hessite, gold, produced in pyrrhotite-polymetallic stage. Three bismuth-tellurides produced in Gaozhuang are well concordant with the standard pilsenite in composition, and other three are different from any of known bismuth-tellurium minerals. Single crystal diffraction were made on a larger mineral grain of pilsenite. Au growth and decline together with Bi and Te in ores and wall-rocks, which suggests that Bi and Te play a important role in migration and enrichment of Au.

The Oscillation Trend and Mechanism of the COD Level of the Liaohe River

CHANG Yuanfei,JIA Zhenbang,ZHAO Zhijie,FANG Zhigang,JIA Yuxia

2002, 38(4):  535-542. 

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The monitoring hydro-data of 8 sections from Fudedian to Zhaoquanhe along the main stream of Liaohe River are collected and analyzed. The Nemero indexes for the water pollution of Liaohe river are calculated and illustrated in diagrams. The results show that the principals of the spatial-temporal COD level changes in the main stream of Liaohe River. Also, two obvious characteristics of COD pollution are found out: (1) the absolute COD level is very high in this river; (2) the ratio of COD/BOD is significantly higher than average. And the phenomena are explained in detail in this paper from multiple aspects such as industrial point pollution, soil and water losses, municipal sewage,agricultural water pollution and etc, based on the relationship analysis between COD, SS and water quality parameters.

Study on the Topographic Variation Pattern of Vegetation Distribution in Wolong Nature Reserve, Sichuan Province

KONG Ningning,ZENG Hui,LI Shujuan

2002, 38(4):  543-549. 

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Two temporal satellite images were used for vegetation mapping of Wolong nature reserve.Through overlay analysis of the vegetation maps and DEM data, the authors studied the distribution pattern variation of different vegetation types along the topographic gradients. The Study resulted that: (1) The extensive deforestation activity beginning at medium 1960's made intensively impact on the vegetation distribution pattern, and the aftereffect can be distinguished clearly even in medium 1980's. (2) The productive afforestation during timber production period from medium 1960's to 1970's and the continually protective afforestation after the setting up of the nature reserve were the primary causes of the significant variation for vegetation distribution pattern along topographic gradients during the study period. Furthermore, natural recovery process also gave an impulse to the pattern changing. (3) Some obvious phenomena of human impact on natural vegetation can also be watched in our working area. Decreasing of coniferous forest and broad-leaved forest with the simultaneous increasing of shrub reflected the type and spatial characteristics of human disturbance activities in working area. The related management department should consider the above problems more seriously.

Gray Geographical Information System-the Theory and Technology of Correct Geological Spatial Data Dynamically

MAO Shanjun

2002, 38(4):  556-562. 

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Concept of GGIS(Gray Geological Information System), together with integrated data model and data structure of 2D-GGIS and TIN, of 3D-GGIS and ARTPN(Analogical Right Triangular Prism Network) are therefore put forward in the paper. It lays a basis for realizing geological data dynamic management. Moreover, the key technology of automatically creating TIN and ARTPN is discussed.

GeoML-Geospatial Information Sharing and Interoperation Language Based on XML

CHEN Aijun,LI Qi,XU Guangyou

2002, 38(4):  550-555. 

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Digital Earth shows great prospects for next generation of Global Information Infrastructure, and the research on geospatial information sharing and interoperation is becoming the frontier of key technology of Digital Earth construction. Based on the OpenGIS standard and the XML standard, Geospatial Object Markup Language-GeoML, which can be used to transmit, display, sharing and interoperation the electronic map on Internet, has been proposed for the first time in this paper. The content of GeoML standard has been detailed, also the prototype system used for testing the standard has been designed and implemented, and the demo is presented. The result shows that publishing, sharing and interoperation of geospatial information on Internet/Intranet have been come into true.

A Study of the Principle and Simulations on Urban Eco-environmental Planning

MAO Feng,MA Qiang,ZOU Jiying,DING Yun

2002, 38(4):  563-571. 

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The authors analyzes the function change of urban in its evolvement and the eco-environmental problems caused by this change. Based on this analysis and the method of systems ecology founded by Odum H T, the authors discuss the aim and the principle of urban eco-environment planning under the sustainable development; put forward the general view of urban eco-environment planning in which the kernel is the adjusting of industry structure, land-use structure and spatial pattern; construct the dynamic simulating model of eco-environment planning of Guangzhou city, China which organizes the models of economy, population, the equilibrium of carbon and oxygen, and other models of atmospheric diffusion, rainwater sedimentation, vegetation absorbing to SO₂, NO_x，TSP；at last simulate the model by means of DYNAMO language and obtain the optimal program of eco-environmental planning of Guangzhou city.

Super-High-Concentration of Glucose Can Accelerate the Senescent Process of Human Diploid Fibroblast Cells

GAO Yunfei,ZHOU Jianjun,CHEN Xi,ZHAO Yun,SU Xiaoqin,LIU Cheng,ZHAI Zhonghe

2002, 38(4):  572-575. 

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Light microtechnique and SA-β-galactosidase method was used to study the effects of super-high-concentration of glucose on the senescence of human diploid fibroblast 2BS cells, ROS and the membrane potential of mitochondria during this process were measured. Our results showed that 200 mmol/L of glucose inhibited the growth of 2BS cells, led to the changes of reactive oxygen species (ROS) and decrease of mitochondrial membrane potential,and caused senescence of 2BS cells rapidly. It supports the hypothesis of oxidative damage of senescence. Moreover it is a better system for the study of the effects of ROS during the process of replicative senescence.

Utilization of Cell Wall of Bamboo Shoot by Wild Giant Panda (Ailuropoda melanoleuca) in Qinling Mountains

LONG Yu,CAO Zhuo,PAN Wenshi

2002, 38(4):  576-581. 

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Giant panda is an indigenous and endangered species in China.It was discussed that the utilization of cell wall of bamboo by giant panda at the morphological viewpoint. The structure of cell wall of Fargesia shoot in both food and feces of giant panda was studied by the ultrathin sectioning and electron microscope. The results showed that the morphological change of the cell wall of Fargesia occurred through giant panda's alimentary canal. The cell wall became thinner, and the rupture and loss of primary and secondary wall was observed. Therefore, it showed that giant panda has the ability to digest part of components of cell wall, such as pectin and semicellulose, etc. Furthermore, it is possible that giant panda can digest a little cellulose. The morphological method used in this study is a new idea for nutrition research. The ability of utilization of cell wall of bamboo by giant panda is unique in carnivore. It is adaptation to its phytophage.

Effects of Recombinant Human Trefoil Factor 3 on the Cell Proliferation and Migration

WANG Yanru,FANG Min,AN Lin,QIAO Wei,LI Lingyuan,RU Binggen

2002, 38(4):  582-586. 

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To investigate the mechanism of trefoil factor 3 on the gastric intestine epithelial restitution, the recombinant human trefoil factor 3 was added to human colonic tumor (HCT) cell and the proliferation effect was examined by MTT assay. The recombinant protein didn't promote the proliferation of the HCT cells at low density of 0.01～0.05 g/L and only has weakly proliferation effect at density of 0.1～0.2 g/L. 1 g/L of the recombinant protein could significantly promote the cell migration of HCT cells when added to the monolayers cells.

The Pilot Study on Arctic Mid-Ocean Ridge——Introduction of AMROE 2001

GAO Yongjun,Jonathon E Snow,MU Zhiguo,SUN Yanrong

2002, 38(4):  587-590. 

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AMORE 2001 has successfully investigated the Gakkel Ridge and Arctic ocean in vicinity of its geological and geophysical nature, as well as the nature of sea ice and biology in this area. The preliminary onboard petrology and bathymetry results show that the mantle beneath the Gakkel ridge with low temperature has only encountered very low degree partial melting during the spreading and there is no any direct relationship between the crust nature and the spreading rate along the Gakkel ridge in any simple petrologic way. Mantle temperature and fracture zone density also play a major role on the crust nature, besides spreading rate.