Abstract: The injection method was used in this paper to investigate the solubilization of BSA in microemulsion of saponified bis(2-ethylhexyl)phosphate (P₂₀₄). When the saponification ratio of P₂₀₄is 87%, the volumefraction (φ_BSA) has the largest value. The concentration of BSA in aqueous phase has no obvious effect on φ_BSAwithin the range ofinves-tigation. However, the increase of the concentration of salts decreases the φ value, and when the salt is CaCl₂, φ_BSAhas its largest decrease. The injection volume is relatively higher within the pH range of 4 to 7. These properties will be helpful in the separation and purification of BSA.

Key words: BSA, microemulsion, bis(2-ethylhexyl)phosphate

摘要： 研究了影响牛血清白蛋白进入皂化二（2-乙基己基）磷酸酯（P₂₀₄）所形成的微乳液水池的因素，发现当萃取剂皂化度为87%时对蛋白的溶解度最大；在所考察的范围内蛋白质的浓度一般无影响；对于盐效应来说，盐浓度增加对蛋白质进入微水池不利，影响程度按NaCl，KCl，CaCl₂的顺序依次增加；对于水相介质条件，当水相pH值为4到7时蛋白质较易进入微乳状液。利用pH和离子强度对蛋白质进入有机相的影响，可以通过调节pH和离子强度来实现萃取和反萃。

关键词: 血清白蛋白, 微乳状液, 二（2-乙基己基）磷酸酯