Abstract: An aerobic Gram-negative bacterium BC001 was isolated from the activated sludge in the coking wastewater treatment plant of Capital Iron and Steel Corporation and identified as Pseudomonas sp. according to 16S rDNA gene sequence analysis and physiological characters. The strain grew well in the high concentrated substrates of pyridine (about 400mg/L) and quinoline (about 500mg/L). For pyridine, the biological removal was only by biosorption, while for quinoline, it included two phases of biosorption and biodegradation. The strain could utilize quinoline as its sole carbon source and nitrogen source, and the biodegradation would be promoted by external carbon source with proper dosage. Main intermediate products in the metabolic pathway of quinoline were detected as 2(1H)-quinolinone and 8-hydroxycoumarin, and NH₄⁺ was confirmed to be the final nitrogen metabolic product.

Key words: pyridine, quinoline, Pseudomonas sp., biosorption, biodegradation

摘要： 从首钢焦化厂废水处理系统的活性污泥中分离出1株能在高浓度的吡啶（约400mg/L）和喹啉（约500mg/L）双基质条件下良好生长的细菌，经16S rDNA及生理形态特征鉴定为假单胞杆菌（Pseudomonas sp. BC001），它对吡啶的去除主要通过生物吸附，而对喹啉的去除包括生物吸附和降解两个阶段。该菌能利用喹啉作为唯一的碳源和氮源代谢生长，适量的外加碳源对喹啉降解具有促进作用，经检测喹啉降解的中间产物主要为2-羟基喹啉和8-羟基香豆素, 氮的主要代谢终产物为NH₄⁺。

关键词: 喹啉, 假单胞杆菌, 生物吸附, 生物降解, 吡啶