Acta Scientiarum Naturalium Universitatis Pekinensis

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Screening Recombinant AcNPV with Methods of Limited Dilution

ZHONG Jin, HU Meihao   

  1. College of Life Sciences, Peking University, Beijing, 100871
  • Received:1997-05-15 Online:1998-07-20 Published:1998-07-20


钟劲, 胡美浩   

  1. 北京大学生命科学学院,北京,100871

Abstract: Viral supernatant of co-transfected sf9 cells is diluted to make cells infected by a single recombinant virus. Because only the recombinant virus express fibrinolysis-active products, it can be screened by measuring activity of medium of transfected sf9 cells. After two turns of screening, we isolated the pure recombinant virus. Compared with the plaque assay usually used in Baculovirus expression system, this method is more convenient and efficient.

Key words: recombinant virus, gradient dilution, screening, activity measurement

关键词: 重组病毒, 梯度稀释, 筛选, 测活

CLC Number: