Abstract: Recent developments on protein engineering of some related proteins in our research are introduced. Specificity change, antiautolysis and role of less conserved disulfide bonds of trypsin are demonstrated. Function of single domain and multi-copied domains of metallothionein is explained. Studies on intra-A chain disulfide bond and C-peptide of insulin, chimera of prourokinase and tissue-type plasminogen activator, mutation of plasminogen activator inhibitor-1, and RGD-containing proteins are also described.

Key words: protein engineering, trypsin, metallothionein, insulin, antithrombotics, protein structure and function, mutant protein, protein engineering, trypsin, metallothionein, insulin, antithrombotics, protein structure and function, mutant protein

摘要： 对近期蛋白质工程的工作进行了综合报道，涉及到胰蛋白酶、金属硫蛋白、胰岛素、尿激酶原、组织型纤溶酶原激活剂、RGD肽等蛋白质或多肽的结构与功能研究以及分子改造。

关键词: 蛋白质工程, 胰蛋白酶, 金属硫蛋白, 胰岛素, 抗凝溶栓剂, 蛋白质结构与功能, 蛋白质突变体, 蛋白质工程, 胰蛋白酶, 金属硫蛋白, 胰岛素, 抗凝溶栓剂, 蛋白质结构与功能, 蛋白质突变体