Acta Scientiarum Naturalium Universitatis Pekinensis

Previous Articles     Next Articles

Kinetics Study of the Cleavage of Cu-thionein Centers in Mammalian CuMT and (Cu, Zn)MT Induced by H2O2

HUAI Qing, FANG Xingwang, WANG Wenqing   

  1. Department of Technical Physics, Peking University, Beijing, 100871
  • Received:1997-04-15 Online:1997-11-20 Published:1997-11-20



  1. 北京大学技术物理学系,北京,100871

Abstract: Typical UV of CuMT scans from 220 to 350nm and exhibits a shoulder near 260nm. Cleavage of Cu-sulfur bond was detected by the decrease of absorbance of 260nm in UV spectra. Kinetics of the H2O2 dependent cleavage of Cu-thiolate centers in copper reconstituted mammalian CuMT was studied. The kinetics type and the possible linear dependency of the rate constants on the H2O2 concentration were performed. The cleavage of Cu-thiolate center in CuMT was compared with that of (Cu, Zn)MT. The results first show that zinc can stabilize the Cu-S chromophore in (Cu, Zn)MT directly.

Key words: metallothionein, H2O2, the cleavage of Cu-S bond

摘要: CuMT紫外光谱的特点是在200~350nm有吸收,在260nm处有一肩峰。260nm的紫外吸收为CuMT中Cu-S键的特征吸收,因此通过观察紫外光谱中CuMT在260nm处吸光度的变化,研究了H2O2引起哺乳动物CuMT中Cu-S键断裂的反应动力学,并且比较了MT中金属组成对反应动力学的影响,结果表明,锌的存在可以稳定CuMT。

关键词: 金属硫蛋白, H2O2, Cu-S键断裂

CLC Number: