Abstract: To establish the gene expression profile of primary gastric carcinoma and lymph node metastasis, isolate and clone gastric metastasis-related genes, the changes of gene expression profile between primary gastric carcinoma and lymph node metastasis were analyzed by cDNA microarray representing approximately 10000 genes and 7000 EST. The differentially expressed genes were analyzed by bioinformaties. Reverse transcription polymerase chain reaction (RT-PCR) and reverse northern dot blot were employed to validate the relationship between primary gastric carcinoma and lymph node metastasis. Subsequently, 601 genes that were differentially expressed in primary gastric carcinoma and lymph node metastasis were identified; 527 genes were highly expressed >2.0-fold in lymph node metastasis, 74 genes were lower expressed >2.0-fold in lymph node metastasis; 71 EST that were differentially expressed in primary gastric carcinoma and lymph node metastasis were identified, 62 EST were highly expressed >2.0-fold in lymph node metastasis,9 EST were low expressed >2.0-fold in lymph node metastasis. In lymph node metastasis, genes related to cell cycle、growth factor、cell motility and cell adhesion were highly expressed. In primary gastric carcinoma, genes related to growth signal and immune response was highly expressed. Furthermore, several genes associated with lymph node metastasis including carbonic anhydrase II and IGFBP-4 wereidentified. These results provide not only a new molecular basis for understanding biological properties of metastasis of gastric carcinoma, but also useful resources for future cloning gastric cancer metastasis-related genes.

Key words: cDNA microarray, gastric carcinoma, metastasis

摘要： 采用cDNA微阵列技术建立胃癌原发灶和淋巴结转移灶基因表达谱，识别和克隆胃癌转移相关基因。用含10000个已知基因和7000个EST的cDNA微阵列分析胃癌原发灶和淋巴结转移灶表达谱的变化，利用生物信息学分析差异表达基因， RT-PCR和反向Northern点杂交验证cDNA微阵列结果。发现2倍以上的差异表达基因601个，其中淋巴结转移灶中表达上调527个，表达下调74个；2倍以上的差异EST 71个，其中淋巴转移灶中表达上调62个，表达下调9个。在胃癌原发灶中，与细胞免疫、发育、信号转导功能相关基因存在高表达；而在淋巴结转移灶中，与细胞生长、细胞周期、细胞运动和粘附功能相关基因存在高表达。RT-PCR和反向Northern点杂交结果进一步证实carbonic anhydraseⅡ、IGFBP-4基因高表达与胃癌转移相关。通过分析胃癌原发灶和淋巴结转移灶表达谱的变化，发现一些与胃癌转移相关的基因和EST，为进一步寻找和克隆胃癌转移相关基因提供研究线索。

关键词: cDNA微阵列, 胃癌, 转移