Abstract: The seasonal changes of peroxidase and estarase isozyme in various tissues of Pinus bungeana were mere related to the annual cycle of cambial activity, and to types of the tissues. During cambial dormancy period, number of peroxidase isozyme bands in cambiumregion reached the maximum. In the time, some cationic estarase isozyme bands occured in the periderm and phloem, these could be related to a protective against frost. When reactitivity of cambium, two cathodic estarase isozyme bands occured in the cambial region, these could be related to enlarging of the cambial cells and formation of new xylem and phloem. During the cambial activity period, othercationic estarase isozyme bands occured in the periderm, xylem andphloem, these could be closely related to the differentiation of phloemand xylem. Some perioxidase isozyme bands disappeared during cambial reactivity, and occured during cambial dormancy period, these could be related to the changes of the level of IAA.

Key words: Pinus bungeana Zucc., periodicity of cambial activity, peroxidase isozyme, estarase isozyme

摘要： 白皮松(Pinus bungeana Zucc.)枝条不同组织中过氧化物酶(POD)和酯酶(EST)同工酶的酶谱也不同，而且每种组织中这两种酶的同工酶酶谱随形成层活动周期的变化而变化。在休眠期，形成层区域过氧化物酶的同工酶酶带最多，这时周皮和韧皮部中酯酶同工酶酶谱在正极端出现一些酶带，这可能与抗寒有关。活动开始时形成层区域负极端出现一特异酯酶酶带，可能与形成层开始分化有关。活动期中，周皮、韧皮部和木质部中酯酶的正极端也有特异性表达的酶带，这些酶带与韧皮部和木质部的分化密切相关。一些过氧化物酶同工酶酶带在形成层恢复活动时消失，到休眠期又出现，这可能与IAA(3-吲哚乙酸)水平的变化有关。

关键词: 白皮松, 形成层活动周期, 酯酶同工酶, 过氧化物酶同工酶